Literature summary for 3.2.1.200 extracted from

Hult, E.L.; Katouno, F.; Uchiyama, T.; Watanabe, T.; Sugiyama, J.
Molecular directionality in crystalline beta-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170 (2005), Biochem. J., 388, 851-856.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Serratia marcescens

Crystallization (Commentary)

Crystallization (Comment)	Organism
ChiB comprises a catalytic (beta/alpha)8 barrel domain and a C-terminal putative chitin-binding domain. The cocrystallized chito-oligosaccharide is inserted into the catalytic cleft from the non-reducing end when digested by ChiB	Serratia marcescens

Organism

Organism	UniProt	Comment	Textmining
Serratia marcescens	P11797	-	-
Serratia marcescens 2170	P11797	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-chitin + H2O	-	Serratia marcescens	?	ChiB is active from the nonreducing end to the reducing end	?
beta-chitin + H2O	-	Serratia marcescens 2170	?	ChiB is active from the nonreducing end to the reducing end	?

Synonyms

Synonyms	Comment	Organism
ChiB	-	Serratia marcescens

General Information

General Information	Comment	Organism
physiological function	digestion of beta-chitin by ChiB occurs from the nonreducing end to the reducing end. Chitinases ChiA and ChiB show synergistic interactions in simultaneous degradation of beta-chitin, but not of glycol chitin	Serratia marcescens

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Release 2023.1 (January 2023)