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Literature summary for 3.2.1.194 extracted from
- Enhanced production of gypenoside LXXV using a novel ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property (2017), Molecules, 22, 844-858 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is applied for the production of gypenoside LXXV from gypenoside XVII at the gram-scale. The pure GypLXXV can be used for the study of anti-cancer effects against three kinds of cancer cells in vitro | Microbacterium sp. Gsoil167 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| BglG167b cloning and overexpression in Escherichia coli as GST-tagged enzyme, phylogenetic analysis of BglG167b | Microbacterium sp. Gsoil167 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Microbacterium sp. Gsoil167 |  |
| Co2+ | - |
Microbacterium sp. Gsoil167 | |
| Cu2+ | - |
Microbacterium sp. Gsoil167 | |
| EDTA | - |
Microbacterium sp. Gsoil167 | |
| SDS | - |
Microbacterium sp. Gsoil167 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity is not affected by Na+, K+, Mg2+, and Mn2+ | Microbacterium sp. Gsoil167 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Re + H2O | Microbacterium sp. Gsoil167 | - |
ginsenoside Rg1 + L-rhamnose | - |
? | |
| ginsenoside Rg1 + H2O | Microbacterium sp. Gsoil167 | - |
ginsenoside F1 + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbacterium sp. Gsoil167 | L0EKQ7 | from ginseng-cultivating soil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography | Microbacterium sp. Gsoil167 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Re + H2O | - |
Microbacterium sp. Gsoil167 | ginsenoside Rg1 + L-rhamnose | - |
? | |
| ginsenoside Rg1 + H2O | - |
Microbacterium sp. Gsoil167 | ginsenoside F1 + D-glucose | - |
? | |
| additional information | enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 | Microbacterium sp. Gsoil167 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90300, about, sequence calculation, x * 116000, about, recombinant GST-tagged enzyme BglG167b, sequence calculation | Microbacterium sp. Gsoil167 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BglG167b | - |
Microbacterium sp. Gsoil167 |
| ginsenoside-transforming beta-glucosidase | - |
Microbacterium sp. Gsoil167 |
| More | cf. EC 3.2.1.194 | Microbacterium sp. Gsoil167 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme | Microbacterium sp. Gsoil167 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | at 30°C and 37°C, the recombinant enzyme has 75.0% and 89.8% of the maximal activity at 45°C | Microbacterium sp. Gsoil167 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 30 | purified recombinant enzyme, 2 h, over 90% activity remaining | Microbacterium sp. Gsoil167 |
| 40 | - |
purified recombinant enzyme, 2 h, over 80% activity remaining | Microbacterium sp. Gsoil167 |
| 45 | - |
purified recombinant enzyme, 2 h, no activity remaining, inactivation above 45°C | Microbacterium sp. Gsoil167 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
recombinant enzyme | Microbacterium sp. Gsoil167 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | over 50% of maximal activity within this range, recombinant enzyme | Microbacterium sp. Gsoil167 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
purified recombinant enzyme, 1.2% activity remaining | Microbacterium sp. Gsoil167 |
| 6 | 9 | purified recombinant enzyme, over 80% activity remaining | Microbacterium sp. Gsoil167 |
| 8.5 | 9 | purified recombinant enzyme, completely stable at | Microbacterium sp. Gsoil167 |
| 10 | - |
purified recombinant enzyme, 40% activity remaining | Microbacterium sp. Gsoil167 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Microbacterium sp. Gsoil167 | sequence calculation | - |
4.56 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme shows homology with glycosyl hydrolases from family 3, GH3 | Microbacterium sp. Gsoil167 |
| metabolism | transformation pathways of ginsenosides by enzyme BglG167b, overview | Microbacterium sp. Gsoil167 |
| additional information | enhanced production of gypenoside LXXV using a ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property. Anti-cancer effects of GypLXXV, Rg3(S), GypXVII and Rb1 on cell viability of HeLa cells, B16 cells, and MDA-MB23 cells | Microbacterium sp. Gsoil167 |
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