Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Gordonia terrae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Gordonia terrae | |
6.61 | - |
ginsenoside Rg1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
6.74 | - |
ginsenoside Re | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
14.66 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
156000 | - |
recombinant His-tagged enzyme, gel filtration | Gordonia terrae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside F1 + H2O | Gordonia terrae | - |
protopanaxatriol + D-glucose | - |
? | |
ginsenoside F1 + H2O | Gordonia terrae DSM 43249 | - |
protopanaxatriol + D-glucose | - |
? | |
ginsenoside F1 + H2O | Gordonia terrae NBRC 100016 | - |
protopanaxatriol + D-glucose | - |
? | |
ginsenoside Re + H2O | Gordonia terrae | - |
ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Re + H2O | Gordonia terrae DSM 43249 | - |
ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Re + H2O | Gordonia terrae NBRC 100016 | - |
ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Rg1 + H2O | Gordonia terrae | - |
ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | Gordonia terrae DSM 43249 | - |
ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | Gordonia terrae NBRC 100016 | - |
ginsenoside Rh1 + D-glucose | - |
? | |
notoginsenoside R1 + H2O | Gordonia terrae | - |
notoginsenoside R2 + D-glucose | - |
? | |
notoginsenoside R1 + H2O | Gordonia terrae DSM 43249 | - |
notoginsenoside R2 + D-glucose | - |
? | |
notoginsenoside R1 + H2O | Gordonia terrae NBRC 100016 | - |
notoginsenoside R2 + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gordonia terrae | - |
- |
- |
Gordonia terrae DSM 43249 | - |
- |
- |
Gordonia terrae NBRC 100016 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Gordonia terrae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
effectivity of ginsenoside production, overview | Gordonia terrae |
1 | - |
purified recombinant enzyme, substrate ginsenoside Rh1, pH 6.5, 30°C | Gordonia terrae |
11 | - |
purified recombinant enzyme, substrate notoginsenoside R1, pH 6.5, 30°C | Gordonia terrae |
11.5 | - |
purified recombinant enzyme, substrate ginsenoside Rg1, pH 6.5, 30°C | Gordonia terrae |
15.9 | - |
purified recombinant enzyme, substrate ginsenoside Re, pH 6.5, 30°C | Gordonia terrae |
132 | - |
purified recombinant enzyme, substrate ginsenoside F1, pH 6.5, 30°C | Gordonia terrae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Gordonia terrae | 2-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Gordonia terrae | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
ginsenoside F1 + H2O | - |
Gordonia terrae | protopanaxatriol + D-glucose | - |
? | |
ginsenoside F1 + H2O | - |
Gordonia terrae DSM 43249 | protopanaxatriol + D-glucose | - |
? | |
ginsenoside F1 + H2O | - |
Gordonia terrae NBRC 100016 | protopanaxatriol + D-glucose | - |
? | |
ginsenoside Re + H2O | - |
Gordonia terrae | ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Re + H2O | - |
Gordonia terrae DSM 43249 | ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Re + H2O | - |
Gordonia terrae NBRC 100016 | ginsenoside Rg2 + L-rhamnose | - |
? | |
ginsenoside Rg1 + H2O | - |
Gordonia terrae | ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | - |
Gordonia terrae DSM 43249 | ginsenoside Rh1 + D-glucose | - |
? | |
ginsenoside Rg1 + H2O | - |
Gordonia terrae NBRC 100016 | ginsenoside Rh1 + D-glucose | - |
? | |
additional information | substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside | Gordonia terrae | ? | - |
? | |
additional information | substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside | Gordonia terrae DSM 43249 | ? | - |
? | |
additional information | substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside | Gordonia terrae NBRC 100016 | ? | - |
? | |
notoginsenoside R1 + H2O | - |
Gordonia terrae | notoginsenoside R2 + D-glucose | - |
? | |
notoginsenoside R1 + H2O | - |
Gordonia terrae DSM 43249 | notoginsenoside R2 + D-glucose | - |
? | |
notoginsenoside R1 + H2O | - |
Gordonia terrae NBRC 100016 | notoginsenoside R2 + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 3.2.1.192 and EC 3.2.1.191 | Gordonia terrae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Gordonia terrae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | activity range, inactive above 50°C, recombinant enzyme | Gordonia terrae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.75 | - |
ginsenoside Rg1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
7.3 | - |
ginsenoside Re | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
6292 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme | Gordonia terrae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
ginsenoside Rg1 | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
1.08 | - |
ginsenoside Re | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae | |
1057.7 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, pH 6.5, 30°C | Gordonia terrae |