Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Fusarium proliferatum | |
Fe2+ | - |
Fusarium proliferatum | |
Fe3+ | - |
Fusarium proliferatum | |
Mn2+ | - |
Fusarium proliferatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slightly activating | Fusarium proliferatum | |
Mg2+ | slightly activating | Fusarium proliferatum | |
additional information | poor effects by Na+, Ca2+, Al3+, Ni2+, and Zn2+ | Fusarium proliferatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Fusarium proliferatum | strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A | ? | - |
? | |
additional information | Fusarium proliferatum WX12 | strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium proliferatum | - |
- |
- |
Fusarium proliferatum WX12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 8.51fold by ammonium sulfate fractionation, ultrafiltration, and anion exchange and hydrophobic interaction chromatography | Fusarium proliferatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the optimum conditions are glucose 10 g/l, yeast 25 g/l, MgSO4 0.3 g/l, NaCl 1.16 g/l, KH2PO 42.72 g/l, pH 6.0, 30°C, resulting in 550 U/ml activity | Fusarium proliferatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified native enzyme shows 37.6 U/ml activity | Fusarium proliferatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + H2O | i.e. sinodiosgenin or trillin | Fusarium proliferatum | D-glucose + L-rhamnose + L-arabinose + diosgenin | - |
? | |
3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + H2O | i.e. sinodiosgenin or trillin | Fusarium proliferatum WX12 | D-glucose + L-rhamnose + L-arabinose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Fusarium proliferatum | D-glucose + 2 L-rhamnose + diosgenin | - |
? | |
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O | - |
Fusarium proliferatum WX12 | D-glucose + 2 L-rhamnose + diosgenin | - |
? | |
additional information | strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A | Fusarium proliferatum | ? | - |
? | |
additional information | enzyme GiGly shows high substrate specificity for multi-3-O-glycosides of spirostanol saponins such as dioscin, trillin and polyphyllin VII, and is inactive toward substrates with terminal groups of rhamnosyl, glucosyl, galactosyl of ginsenoside Re and saikosaponin A. It can only hydrolyze glycosidic bonds at the C-3 position on steroidal saponins, which have similar structure with dioscin, and can be transformed into diosgenin. Substrate specificity, overview. GiGly is able tohydrolyze the terminal alpha-1,2-linked rhamnosyl residues, alpha-1,4-linked rhamnosyl residues, alpha-1,4-linked arabinosyl residues and beta-D-glucosyl residues at C-3 position.. The hydrolyzed substrates of GiGly have a double bond between carbons 5 and 6 position. By contrast, the non-substrates lack the said double bond and have substituents at carbon 4 position | Fusarium proliferatum | ? | - |
? | |
additional information | strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A | Fusarium proliferatum WX12 | ? | - |
? | |
additional information | enzyme GiGly shows high substrate specificity for multi-3-O-glycosides of spirostanol saponins such as dioscin, trillin and polyphyllin VII, and is inactive toward substrates with terminal groups of rhamnosyl, glucosyl, galactosyl of ginsenoside Re and saikosaponin A. It can only hydrolyze glycosidic bonds at the C-3 position on steroidal saponins, which have similar structure with dioscin, and can be transformed into diosgenin. Substrate specificity, overview. GiGly is able tohydrolyze the terminal alpha-1,2-linked rhamnosyl residues, alpha-1,4-linked rhamnosyl residues, alpha-1,4-linked arabinosyl residues and beta-D-glucosyl residues at C-3 position.. The hydrolyzed substrates of GiGly have a double bond between carbons 5 and 6 position. By contrast, the non-substrates lack the said double bond and have substituents at carbon 4 position | Fusarium proliferatum WX12 | ? | - |
? | |
polyphyllin VII + 4 H2O | - |
Fusarium proliferatum | 3 L-rhamnose + D-glucose + (22zeta,25R)-9zeta-spirost-5-en-3beta,17-diol | - |
? | |
polyphyllin VII + 4 H2O | - |
Fusarium proliferatum WX12 | 3 L-rhamnose + D-glucose + (22zeta,25R)-9zeta-spirost-5-en-3beta,17-diol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Fusarium proliferatum |
More | peptide mass fingerprinting, analysis through mass spectrometry and SDS-PAGE | Fusarium proliferatum |
Synonyms | Comment | Organism |
---|---|---|
GiGly | - |
Fusarium proliferatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Fusarium proliferatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purified native enzyme GiGly conserved more than 70% of the original activity after 10 h of incubation at 4°C | Fusarium proliferatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Fusarium proliferatum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | purified enzyme GiGly, stable at pH 5.0-8.0 retaining 80% activity at pH 7.0 after 12 h | Fusarium proliferatum |