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Literature summary for 3.2.1.186 extracted from

  • Suthangkornkul, R.; Sriworanun, P.; Nakai, H.; Okuyama, M.; Svasti, J.; Kimura, A.; Senapin, S.; Arthan, D.
    A Solanum torvum GH3 beta-glucosidase expressed in Pichia pastoris catalyzes the hydrolysis of furostanol glycoside (2016), Phytochemistry, 127, 4-11 .
    View publication on PubMed
Search for more literature for 3.2.1.186

Cloned(Commentary)

Cloned (Comment) Organism
gene bgl3, DNA and amino acid sequence determiantion and analysis, sequence comparisons, quantitative real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Pichia pastoris with post-translational glycosylation Solanum torvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 torvoside A native enzyme, pH 5.0, 37°C Solanum torvum
0.069
-
 torvoside A recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
0.66
-
 4-methylumbelliferyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
0.78
-
 4-methylumbelliferyl beta-D-glucoside native enzyme, pH 5.0, 37°C Solanum torvum
1.01
-
 4-nitrophenyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
1.03
-
 4-nitrophenyl beta-D-glucoside native enzyme, pH 5.0, 37°C Solanum torvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 native enzyme, gel filtration Solanum torvum
169500
-
 glycosylated recombinant His-tagged enzyme, gel filtration Solanum torvum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum torvum the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H ?
-
 ?
torvoside A + H2O Solanum torvum
-
 26-deglucotorvoside A + D-glucose
-
 ?
torvoside H + H2O Solanum torvum
-
 26-deglucotorvoside H + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum torvum P0DKH4 i.e. Solanum ficifolium
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the native enzyme is glycosylated, while the recombinant enzyme is hyperglycosylated, deglycosylation by endoglycosidase H (EndoH) shifting the molecular weight from 140 to 70 kDa Solanum torvum

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 323fold from Pichia pastoris by nickel affinity chromatography Solanum torvum

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression level Solanum torvum
-
additional information quantitative real-time PCR expression analysis Solanum torvum
-
seed germinating, high expression level Solanum torvum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
 purified recombinant His-tagged enzyme, substrate torvoside A, pH 5.0, 37°C Solanum torvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Solanum torvum 4-methylumbelliferol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Solanum torvum 4-nitrophenol + beta-D-glucose
-
 ?
decyl-beta-D-glucoside + H2O 83% activity compared to torvoside A Solanum torvum decanol + D-glucose
-
 ?
hexyl-beta-D-glucoside + H2O 43% activity compared to torvoside A Solanum torvum hexanol + D-glucose
-
 ?
additional information the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H Solanum torvum ?
-
 ?
additional information the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H. No activity with (1,2)-beta-linked glucobiose and (1,3)-beta-linked glucobiose, and with beta-linked oligosaccharides, namely cellobiose, cellotriose, cellotetraose, laminaribiose, laminaritriose, laminaritetraose, and gentiobiose Solanum torvum ?
-
 ?
octyl-beta-D-glucoside + H2O 69% activity compared to torvoside A Solanum torvum octanol + D-glucose
-
 ?
phenyl-beta-D-glucoside + H2O 36% activity compared to torvoside A Solanum torvum phenol + D-glucose
-
 ?
torvoside A + H2O
-
 Solanum torvum 26-deglucotorvoside A + D-glucose
-
 ?
torvoside A + H2O best substrate for native and recombinant enzymes Solanum torvum 26-deglucotorvoside A + D-glucose
-
 ?
torvoside H + H2O
-
 Solanum torvum 26-deglucotorvoside H + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66573, sequence calculation, 1 * 80700, native enzyme, SDS-PAGE, 1 * 140000, glycosylated recombinant His-tagged enzyme, SDS-PAGE, 1 * 70000, deglycosylated recombinant His-tagged enzyme, SDS-PAGE Solanum torvum

Synonyms

Synonyms Comment Organism
S. torvum beta-glucosidase
-
 Solanum torvum
SBgl3
-
 Solanum torvum
Solanum torvum GH3 beta-glucosidase
-
 Solanum torvum
torvosidase
-
 Solanum torvum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Solanum torvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 4-methylumbelliferyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
4.9
-
 4-nitrophenyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
7.3
-
 torvoside A recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
8.3
-
 4-methylumbelliferyl beta-D-glucoside native enzyme, pH 5.0, 37°C Solanum torvum
8.6
-
 torvoside A native enzyme, pH 5.0, 37°C Solanum torvum
9.1
-
 4-nitrophenyl beta-D-glucoside native enzyme, pH 5.0, 37°C Solanum torvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 recombinant His-tagged enzyme Solanum torvum
5
-
 native enzyme Solanum torvum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum torvum sequence calculation
-
 8.07

General Information

General Information Comment Organism
evolution torvosidase is classified as a glycosyl hydrolase family GH3 beta-glucosidase, but cannot hydrolyze beta-oligoglucosides, the natural substratesof GH3 enzymes. Its substrates, furostanol glycoside 26-O-beta-glucosides, are reported as natural substrates of some plant GH1 enzymes Solanum torvum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.85
-
 4-nitrophenyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
7.12
-
 4-methylumbelliferyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
8.8
-
 4-nitrophenyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
10.6
-
 4-methylumbelliferyl beta-D-glucoside recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
105.8
-
 torvoside A recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum
136.5
-
 torvoside A recombinant His-tagged enzyme, pH 5.0, 37°C Solanum torvum