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Literature summary for 3.2.1.174 extracted from
- Rhamnogalacturonan alpha-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin (1994), Plant Physiol., 106, 241-250.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sodium acetate | 0.1 M slowly inactivates the enzyme: at 0.5 M 33% residual activity, at 1.1 M 10% residual activity | Aspergillus aculeatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
x * 84000, SDS-PAGE | Aspergillus aculeatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rhamnogalacturonan I oligosaccharides with D-galacturonic acid at the reducing end and L-rhamnopyranose at the nonreducing end + H2O | alpha-L-Rha-(1->4)-[alpha-D-GalA-(1->2)-alpha-L-Rha]n-(1->4)-alpha-D-GalA. The enzyme is hindered when the terminal Rha residue is substituted at the 4-position by a beta-D-galactose. No particular preference of the enzyme for low or high molecular mass rhamnogalacturonan fragments | Aspergillus aculeatus | beta-L-rhamnopyranose + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing end | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, SDS-PAGE | Aspergillus aculeatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RG alpha-L-rhamnopyranohydrolase | - |
Aspergillus aculeatus |
| RG-rhamnohydrolase | - |
Aspergillus aculeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
sodium acetate buffer | Aspergillus aculeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 1 h up to temperatures of 60°C in 50 mM sodium acetate buffer (pH 5) | Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
McIlvaine buffer | Aspergillus aculeatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | stable | Aspergillus aculeatus |
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Release 2023.1 (January 2023)
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