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Literature summary for 3.2.1.166 extracted from

  • Bohdan, N.; Espin, S.; Aguila, S.; Teruel-Montoya, R.; Vicente, V.; Corral, J.; Martinez-Martinez, I.
    Heparanase activates antithrombin through the binding to its heparin binding site (2016), PLoS ONE, 11, e0157834 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.166

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of myc-DDK-tagged proheparanase in HEK-293T cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular matrix
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 Homo sapiens 31012
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens heparanase, but not proheparanase, interacts directly with antithrombin in a non-covalent manner resulting in the activation of antithrombin. Complex formation and activity analysis, overview. The activation of Pro41Leu, Arg47Cys, Lys114Ala and Lys125Ala antithrombin mutants by heparanase is impaired ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y251
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Purification (Commentary)

Purification (Comment) Organism
recombinant myc-DDK-tagged proheparanase partially by tangential ultrafiltration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation purified recombinant detagged enzyme Homo sapiens
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endothelium the enzyme penetrates the endothelial layer that lines blood vessels Homo sapiens
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additional information tumor cell Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information heparanase, but not proheparanase, interacts directly with antithrombin in a non-covalent manner resulting in the activation of antithrombin. Complex formation and activity analysis, overview. The activation of Pro41Leu, Arg47Cys, Lys114Ala and Lys125Ala antithrombin mutants by heparanase is impaired Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
HPSE
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 Homo sapiens

General Information

General Information Comment Organism
physiological function heparanase is an endoglycosidase that participates in morphogenesis, tissue repair, heparan sulphates turnover and immune response processes. It is overexpressed in tumor cells favoring the metastasis as it penetrates the endothelial layer that lines blood vessels and facilitates the metastasis by degradation of heparan sulphate proteoglycans of the extracellular matrix. Heparanase may also affect the hemostatic system in a non-enzymatic manner, up-regulating the expression of tissue factor, which is the initiator of blood coagulation, and dissociating tissue factor pathway inhibitor on the cell surface membrane of endothelial and tumor cells, thus resulting in a procoagulant state. Heparanase activates antithrombin through the binding to its heparin binding site. Activation of antithrombin, which is the most important endogenous anticoagulant, mainly accelerates factor Xa inhibition, supporting an allosteric activation effect. Heparanase may exert a non-enzymatic function interaction Homo sapiens