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Literature summary for 3.2.1.165 extracted from

  • Dennhart, N.; Fukamizo, T.; Brzezinski, R.; Lacombe-Harvey, M.E.; Letzel, T.
    Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry (2008), J. Biotechnol., 134, 253-260.
    View publication on PubMed
Search for more literature for 3.2.1.165

Cloned(Commentary)

Cloned (Comment) Organism
Streptomyces lividans TK-24 with shuttle vector pFD666, M14 medium, pH 4.2 Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Amycolatopsis orientalis Chitosanases hydrolyze beta-1,4-glycosidic linkage of chitosan, a polysaccharide consisting mainly of D-glucosamine with a variable content of N-acetyl-D-glucosamine ?
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 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis
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Purification (Commentary)

Purification (Comment) Organism
SP-Sepharose ion exchange chromatography followed by hydroxyapatite column chromatography Amycolatopsis orientalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 real-time MS as high sensitivity method to measure chitosanase activity quickly and with low substrate concentration, simultaneous detection of substrate and product Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O GlcNn: polymerization degree of n=2-6, chitosan oligosaccharides, 0.96 nM exochiosanase, 10 mM ammonium acetate, pH 5.2, substrate concentrations: 6.25, 12.5, 25, 37.5 microM, hexa-N-acetylchitohexaose as control substrate at 25 microM Amycolatopsis orientalis 2-amino-2-deoxy-D-glucopyranose + chitopentaose
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 ?
additional information Chitosanases hydrolyze beta-1,4-glycosidic linkage of chitosan, a polysaccharide consisting mainly of D-glucosamine with a variable content of N-acetyl-D-glucosamine Amycolatopsis orientalis ?
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 ?

Synonyms

Synonyms Comment Organism
exo-beta-glucosaminidase
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 Amycolatopsis orientalis
exochitosanase
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 Amycolatopsis orientalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
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 chitohexaose 2-amino-2-deoxy-D-glucopyranose split off, pH 5.2, 20°C Amycolatopsis orientalis