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Literature summary for 3.2.1.165 extracted from

  • Zhang, X.Y.; Dai, A.L.; Zhang, X.K.; Kuroiwa, K.; Kodaira, R.; Shimosaka, M.; Okazaki, M.
    Purification and characterization of chitosanase and exo-beta-D-glucosaminidase from a Koji mold, Aspergillus oryzae IAM2660 (2000), Biosci. Biotechnol. Biochem., 64, 1896-1902.
    View publication on PubMed
Search for more literature for 3.2.1.165

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae IAM2660
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.8
-
-
 Aspergillus oryzae

Storage Stability

Storage Stability Organism
4°C, pH 5.5, sodium acetate buffer, 4 months, stable Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae D-glucosamine + ?
-
 ?
chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae IAM2660 D-glucosamine + ?
-
 ?
chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae D-glucosamine + ?
-
 ?
chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae IAM2660 D-glucosamine + ?
-
 ?
glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae ?
-
 ?
glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae IAM2660 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 135000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
exo-beta-D-glucosaminidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 20 min, 30% loss of activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus oryzae