Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.152 extracted from

  • Ishimizu, T.; Hashimoto, C.; Takeda, R.; Fujii, K.; Hase, S.
    A novel {alpha}1,2-L-Fucosidase acting on xyloglucan oligosaccharides is associated with endo-beta-mannosidase (2007), J. Biochem., 142, 721-729.
    View publication on PubMed
Search for more literature for 3.2.1.152

Organism

Organism UniProt Comment Textmining
Lilium longiflorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme is purified by ammonium sulfate precipitation, DEAE-Sephacel chromatography, sephacryl S-200 gel filtration, octyl sepharose CL-4B chromatography and superdex 200 gel filtration Lilium longiflorum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Lilium longiflorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man-alpha-(1-3)Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O relative hydrolysis rate: 42% Lilium longiflorum GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-3)Man-alpha-(1-6)Man
-
 ?
Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O relative hydrolysis rate: 34% Lilium longiflorum GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man
-
 ?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O relative hydrolysis rate: 100% Lilium longiflorum GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
-
 ?
Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine + H2O relative hydrolysis rate: 9% Lilium longiflorum D-mannose + GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine
-
 ?
additional information EBM II has both endo-beta-mannosidase activity and alpha 1,2-L-fucosidase activity Lilium longiflorum ?
-
 ?

Subunits

Subunits Comment Organism
More SDS-PAGE of the purified protein shows that EBM II is comprised of six polypeptides with molecular masses of 65000, 46000, 42000, 31000, 28000 and 27000 Da. Analysis of the fragments disclose EBM II consists of endo-beta-mannosidase and an associated protein that has slight homology to the fucosidase domain of the bifidus AfcA protein Lilium longiflorum

Synonyms

Synonyms Comment Organism
EBM II
-
 Lilium longiflorum
endo-beta-mannosidase
-
 Lilium longiflorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Lilium longiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Lilium longiflorum