KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | in ammonium acetate buffer, pH 5.0, at 37°C | Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration | Brassica oleracea |
28000 | - |
1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration | Brassica oleracea |
34000 | - |
1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration | Brassica oleracea |
42000 | - |
1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration | Brassica oleracea |
141000 | - |
SDS-PAGE | Brassica oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica oleracea |
ammonium sulfate precipitation, Poros HS column chromatography, hydroxyapatite chromatography, DEAE-Sephacel column chromatography, Octyl Sepharose CL-4B column chromatography, and Superdex 200 gel filtration | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O | - |
Brassica oleracea | Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide | - |
? | |
Man-alpha-(1-6)Man-alpha(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O | - |
Brassica oleracea | Man-alpha-(1-6)Man-alpha(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide | - |
? | |
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O | - |
Brassica oleracea | Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide | - |
? | |
Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 40% activity relative to M2B-PA | Brassica oleracea | Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | 34% activity relative to Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Brassica oleracea | Manalpha(1-3)[Manalpha(1-6)]Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | 100% activity | Brassica oleracea | Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | 2.6% activity relative to M2B-PA | Brassica oleracea | beta-D-mannose + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
additional information | Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[(Manalpha(1-3))]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, GlcNAcbeta(1-4)Manalpha(1-3)[GlyNAcbeta(1-4)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-6)[Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))]GlcNAc-pyridylaminoside, p-nitrophenyl alpha-mannoside, p-nitrophenyl beta-mannoside, and Manbeta-(1-4)Manbeta(1-4)Man-beta-(1-4)Manbeta(1-4)Man-beta(1-4)Man are no substrates | Brassica oleracea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration | Brassica oleracea |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-mannosidase | - |
Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Brassica oleracea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | relatively stable at pH 3-6 | Brassica oleracea |