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Literature summary for 3.2.1.152 extracted from
- Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization (2004), J. Biol. Chem., 279, 38555-38562.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli RosettaBlue | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E464A | no activity | Arabidopsis thaliana |
| E549A | no activity | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
gel filtration | Lilium longiflorum |
| 112000 | - |
- |
Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man-beta-(1-4)GlcNAc + H2O | Lilium longiflorum | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | ? | - |
? |  |
| Man-beta-(1-4)GlcNAc + H2O | Arabidopsis thaliana | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q75W54 | - |
- |
| Lilium longiflorum | - |
Thunb. cv Hinomoto | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lilium longiflorum |
| partial purification of the recombinant protein | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Lilium longiflorum | - |
| flower | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.026 | - |
purified enzyme, pH 5.0, 37°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | - |
Lilium longiflorum | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man | - |
? | |
| Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | - |
Arabidopsis thaliana | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man | - |
? | |
| Man-beta-(1-4)GlcNAc + H2O | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | Lilium longiflorum | ? | - |
? | |
| Man-beta-(1-4)GlcNAc + H2O | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside | Lilium longiflorum | ? | - |
? | |
| additional information | enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | three bands on SDS-PAGE of 42000, 31000, and 28000 Da | Lilium longiflorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-mannosidase | - |
Lilium longiflorum |
| endo-beta-mannosidase | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Arabidopsis thaliana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | unstable below pH 3 and above pH 7 | Arabidopsis thaliana |
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