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Literature summary for 3.2.1.15 extracted from
- Purification and mechanistic characterization of two polygalacturonases from Sclerotium rolfsii (2007), Enzyme Microb. Technol., 40, 1739-1747.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strongly inhibits PG1 and PG2 | Athelia rolfsii |  |
| Cr3+ | stronger inhibitor for PG2 than for PG1 | Athelia rolfsii | |
| Fe3+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
| Hg2+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
| Mg2+ | - |
Athelia rolfsii | |
| additional information | EDTA and surfactants have no influence on enzyme activity | Athelia rolfsii | |
| SDS | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
PG2 | Athelia rolfsii |
| 39500 | - |
PG1 | Athelia rolfsii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Athelia rolfsii | - |
- |
- |
| Athelia rolfsii CBS 350.80 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, by free flow electrophoresis in isoelectric focusing mode using precipitation, followed by gel filtration | Athelia rolfsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii | ? | - |
? | |
| additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii CBS 350.80 | ? | - |
? | |
| pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
| pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
| polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
| polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
| [beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
| [beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
| [beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii | ? | - |
? | |
| [beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii CBS 350.80 | ? | - |
? | |
| [beta-D-GalA(1-4)]5 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG1 | - |
Athelia rolfsii |
| PG2 | - |
Athelia rolfsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
isoenzyme PG2 | Athelia rolfsii |
| 60 | - |
isoenzyme PG1 | Athelia rolfsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
PG1 is stable at 50°C, at 70°C stability is drastically reduced. PG2 is a very sensitive enzyme, its half-life time at 50°C is in the range of min and at 70°C the enzyme is inactivated within 1 min | Athelia rolfsii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
isoenzyme PG2 | Athelia rolfsii |
| 5 | - |
isoenzyme PG1 | Athelia rolfsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
PG1 is stable at pH 5, exhibits more sensitivity against pH values below and above 5 | Athelia rolfsii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Athelia rolfsii | PG2 | - |
5.4 |
| Athelia rolfsii | PG1 | - |
6.5 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
isoenzyme PG1 | Athelia rolfsii | Hg2+ | |
| 0.5 | - |
isoenzyme PG1 | Athelia rolfsii | SDS | |
| 0.75 | - |
isoenzyme PG1 | Athelia rolfsii | Fe3+ | |
| 0.9 | - |
isoenzyme PG2 | Athelia rolfsii | Hg2+ | |
| 5 | - |
isoenzyme PG1 and PG2 | Athelia rolfsii | Mg2+ | |
| 7 | - |
isoenzyme PG1 | Athelia rolfsii | Ca2+ | |
| 12 | - |
isoenzyme PG2 | Athelia rolfsii | SDS | |
| 15 | - |
isoenzyme PG2 | Athelia rolfsii | Ca2+ | |
| 16 | - |
isoenzyme PG2 | Athelia rolfsii | Fe3+ | |
| 18 | - |
isoenzyme PG2 | Athelia rolfsii | Cr3+ | |
| 32 | - |
isoenzyme PG1 | Athelia rolfsii | Cr3+ | |
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