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Literature summary for 3.2.1.15 extracted from
- Active site characterization of the single endo-polygalacturonase produced by Fusarium moniliforme NCIM 1276 (2001), Eur. J. Biochem., 268, 832-840.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | 50 mM, 70% inhibition | Fusarium verticillioides |  |
| 2,3-Butanedione | 10 mM, 90% inhibition | Fusarium verticillioides | |
| 2-Hydroxy-5-nitrobenzylbromide | 10 mM, complete inhibition | Fusarium verticillioides | |
| diethyl dicarbonate | 5 mM, complete inhibition | Fusarium verticillioides | |
| gluconic acid delta-lactone | compeptitive | Fusarium verticillioides | |
| N-bromosuccinimide | 1 mM, complete inhibition | Fusarium verticillioides | |
| p-nitrophenylglyoxal | 5 mM, complete inhibition | Fusarium verticillioides | |
| Phenylglyoxal | 5 mM, complete inhibition | Fusarium verticillioides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for polygalacturonate is 0.12 mg/ml, at pH 4.8, 45°C | Fusarium verticillioides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium verticillioides | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Fusarium verticillioides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium verticillioides | - |
- |
- |
| Fusarium verticillioides NCIM 1276 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content is 4% | Fusarium verticillioides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium verticillioides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 178.6 | - |
- |
Fusarium verticillioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Fusarium verticillioides | ? | - |
? | |
| polygalacturonate + H2O | - |
Fusarium verticillioides NCIM 1276 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Fusarium verticillioides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-polygalacturonase | - |
Fusarium verticillioides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70 | - |
Polygalacturonate | pH 4.8, 45°C | Fusarium verticillioides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Fusarium verticillioides |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28 | - |
gluconic acid delta-lactone | pH 4.8, 45°C | Fusarium verticillioides | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusarium verticillioides | - |
- |
6.9 |
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