General Stability | Organism |
---|---|
Ca2+ stabilizes | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inactivation | Bacillus sp. (in: Bacteria) | |
NaCl | 0.3 M, complete inactivation | Bacillus sp. (in: Bacteria) | |
PCMB | 0.1 M, complete inactivation | Bacillus sp. (in: Bacteria) | |
Urea | 8 M, complete inactivation | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal activation at 1 mM | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 70000 | gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
No. P-4-N | - |
Bacillus sp. (in: Bacteria) No. P-4-N | - |
No. P-4-N | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectic acid + H2O | - |
Bacillus sp. (in: Bacteria) | galacturonic acid oligomers | - |
? | |
pectic acid + H2O | - |
Bacillus sp. (in: Bacteria) No. P-4-N | galacturonic acid oligomers | - |
? | |
polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | oligogalacturonates | liberation of digalacturonic acid, trigalacturonic acid and tetragalacturonic acid after 10 min. After 2 h, tetragalacturonic acid disappears with concomitant accumulation of galacturonate | ? | |
polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) No. P-4-N | oligogalacturonates | liberation of digalacturonic acid, trigalacturonic acid and tetragalacturonic acid after 10 min. After 2 h, tetragalacturonic acid disappears with concomitant accumulation of galacturonate | ? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 30% of maximal activity, 70°C: about 70% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
5 min, 80% of the activity remains | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.5 | - |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 12 | pH 8.5: about 30% of maximal activity, pH 12.0: about 40% of maximal activity | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
70°C, 10 min, 40% loss of activity | Bacillus sp. (in: Bacteria) |
6.5 | - |
70°C, 10 min, most stable at | Bacillus sp. (in: Bacteria) |
11 | - |
70°C, 10 min, 70% loss of activity | Bacillus sp. (in: Bacteria) |