BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Characterization of an exo-beta-1,3-D-galactanase from Sphingomonas sp. 24T and its application to structural analysis of larch wood arabinogalactan

Sakamoto, T.; Tanaka, H.; Nishimura, Y.; Ishimaru, M.; Kasai, N.; Appl. Microbiol. Biotechnol. 90, 1701-1710 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
addition of galactose at a concentration of 2 mM to the reaction mixture does not affect the enzyme activity
Sphingomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
x * 48000, SDS-PAGE
Sphingomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Sphingomonas sp.
-
-
-
Sphingomonas sp. 24T
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography
Sphingomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.88
-
culture filtrate, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C
Sphingomonas sp.
1.7
-
after 1.9fold purification, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C
Sphingomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-(1,3)-D-galactooligosaccharides + H2O
-
713904
Sphingomonas sp.
D-galactose + ?
-
-
-
?
beta-(1,3)-D-galactooligosaccharides + H2O
-
713904
Sphingomonas sp. 24T
D-galactose + ?
-
-
-
?
beta-(1,3)-galactobiose + H2O
-
713904
Sphingomonas sp.
D-galactose
-
-
-
?
beta-(1,3)-galactobiose + H2O
-
713904
Sphingomonas sp. 24T
D-galactose
-
-
-
?
beta-(1-3)-D-galactan + H2O
the enzyme releases approximately 60% of the total D-galactose in the substrate
713904
Sphingomonas sp.
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-(1-3)-D-galactan + H2O
the enzyme releases approximately 60% of the total D-galactose in the substrate
713904
Sphingomonas sp. 24T
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-1,3-galactotriose + H2O
-
713904
Sphingomonas sp.
D-galactose + beta-(1,3)-galactobiose
-
-
-
?
beta-1,3-galactotriose + H2O
-
713904
Sphingomonas sp. 24T
D-galactose + beta-(1,3)-galactobiose
-
-
-
?
gum arabic + H2O
weak substrate
713904
Sphingomonas sp.
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme bypasses the branching points of beta-1,3-galactan backbones in larch wood arabinogalactan
713904
Sphingomonas sp.
D-galactose + beta-(1->6)-D-galactobiose + beta-(1->6)-D-galactotriose + O-alpha-L-arabinofuranosyl-(1->3)-O-beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + O-beta-D-galactopyranosyl-(1->6)-[O-alpha-L-arabinofuranosyl-(1->3)-]-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + ?
-
-
-
?
additional information
no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages
713904
Sphingomonas sp.
?
-
-
-
-
additional information
no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages
713904
Sphingomonas sp. 24T
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 48000, SDS-PAGE
Sphingomonas sp.
Synonyms
Synonyms
Commentary
Organism
Exo-1,3-Gal
-
Sphingomonas sp.
exo-beta-1,3-D-galactanase
-
Sphingomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
the enzyme is most active around 50°C
Sphingomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
the enzyme is stable at temperatures up to 40°C
Sphingomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
at 37°C
Sphingomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
10
more than 80% of the initial activity remains after 16 h of incubation at pH values from 3.0 to 10.0
Sphingomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
addition of galactose at a concentration of 2 mM to the reaction mixture does not affect the enzyme activity
Sphingomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
x * 48000, SDS-PAGE
Sphingomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography
Sphingomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.88
-
culture filtrate, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C
Sphingomonas sp.
1.7
-
after 1.9fold purification, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C
Sphingomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-(1,3)-D-galactooligosaccharides + H2O
-
713904
Sphingomonas sp.
D-galactose + ?
-
-
-
?
beta-(1,3)-D-galactooligosaccharides + H2O
-
713904
Sphingomonas sp. 24T
D-galactose + ?
-
-
-
?
beta-(1,3)-galactobiose + H2O
-
713904
Sphingomonas sp.
D-galactose
-
-
-
?
beta-(1,3)-galactobiose + H2O
-
713904
Sphingomonas sp. 24T
D-galactose
-
-
-
?
beta-(1-3)-D-galactan + H2O
the enzyme releases approximately 60% of the total D-galactose in the substrate
713904
Sphingomonas sp.
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-(1-3)-D-galactan + H2O
the enzyme releases approximately 60% of the total D-galactose in the substrate
713904
Sphingomonas sp. 24T
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-1,3-galactotriose + H2O
-
713904
Sphingomonas sp.
D-galactose + beta-(1,3)-galactobiose
-
-
-
?
beta-1,3-galactotriose + H2O
-
713904
Sphingomonas sp. 24T
D-galactose + beta-(1,3)-galactobiose
-
-
-
?
gum arabic + H2O
weak substrate
713904
Sphingomonas sp.
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme bypasses the branching points of beta-1,3-galactan backbones in larch wood arabinogalactan
713904
Sphingomonas sp.
D-galactose + beta-(1->6)-D-galactobiose + beta-(1->6)-D-galactotriose + O-alpha-L-arabinofuranosyl-(1->3)-O-beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + O-beta-D-galactopyranosyl-(1->6)-[O-alpha-L-arabinofuranosyl-(1->3)-]-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + ?
-
-
-
?
additional information
no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages
713904
Sphingomonas sp.
?
-
-
-
-
additional information
no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages
713904
Sphingomonas sp. 24T
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 48000, SDS-PAGE
Sphingomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
the enzyme is most active around 50°C
Sphingomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
the enzyme is stable at temperatures up to 40°C
Sphingomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
at 37°C
Sphingomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
10
more than 80% of the initial activity remains after 16 h of incubation at pH values from 3.0 to 10.0
Sphingomonas sp.
Other publictions for EC 3.2.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
Degradative enzymes for type ...
Bifidobacterium longum subsp. Longum
Appl. Microbiol. Biotechnol.
103
1299-1310
2019
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
-
-
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
9
-
14
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
5
-
2
2
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
10
1
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
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171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
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