BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Molecular cloning of a b-galactosidase from radish that specifically hydrolyzes beta-(1->3)- and beta-(1->6)-galactosyl residues of arabinogalactan protein

Kotake, T.; Dina, S.; Konishi, T.; Kaneko, S.; Igarashi, K.; Samejima, M.; Watanabe, Y.; Kimura, K.; Tsumuraya, Y.; Plant Physiol. 138, 1563-1576 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Raphanus sativus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
1 mM, almost complete inhibition
Raphanus sativus
iodoacetic acid
1 mM, about 80% inhibition
Raphanus sativus
additional information
not inhibitory: Mn2+, Fe3+, Cu2+, Mg2+, Ba2+, Ca2+, Co2+, Zn2+, Cd2+
Raphanus sativus
sodium dodecylsulfate
1 mM, about 80% inhibition
Raphanus sativus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
p-nitrophenyl-beta-galactoside
pH 4.0, 37C
Raphanus sativus
0.77
-
beta-(1-3)-D-galactotriose
pH 4.0, 37C
Raphanus sativus
1.77
-
beta-(1-3)-galactobiose
pH 4.0, 37C
Raphanus sativus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33654
-
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
44981
-
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
Organism
Organism
UniProt
Commentary
Textmining
Raphanus sativus
Q6L619
recombinant enzyme
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant enzyme expressed in Pichia pastoris
Raphanus sativus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
64.9
-
pH 4.0, 37C
Raphanus sativus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-(1-3)-D-galactan + H2O
8% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-(1-3)-D-galactotriose + H2O
44% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-3)-D-galactobiose + D-galactose
-
-
-
?
beta-(1-3)-galactobiose + H2O
32% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
D-galactose
-
-
-
?
beta-(1-6)-galactopentaose + H2O
50% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactotetraose + D-galactose
-
-
-
?
beta-(1-6)-galactotetraose + H2O
68% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactotriose + D-galactose
-
-
-
?
beta-(1-6)-galactotriose + H2O
49% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactobiose + D-galactose
-
-
-
?
beta-galactosido-(1-3)-beta-galactosido-(1-6)-galactoside + H2O
42% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-galactosido-(1-3)-beta-galactoside + D-galactose
-
-
-
-
beta-galactosido-(1-6)-beta-galactosido-(1-3)-galactoside + H2O
40% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-galactosido-(1-6)-beta-galactoside + D-galactose
-
-
-
-
additional information
specific hydrolysis of beta-1,3 and beta-1,6 galactooligosaccharides
666634
Raphanus sativus
?
-
-
-
-
p-nitrophenyl-beta-galactoside + H2O
-
666634
Raphanus sativus
p-nitrophenol + beta-galactose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
Synonyms
Synonyms
Commentary
Organism
RsBGAL1
-
Raphanus sativus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Raphanus sativus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
65
-
loss of 90% of activity
Raphanus sativus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
28.6
-
beta-(1-3)-galactobiose
pH 4.0, 37C
Raphanus sativus
32.5
-
beta-(1-3)-D-galactotriose
pH 4.0, 37C
Raphanus sativus
123
-
p-nitrophenyl-beta-galactoside
pH 4.0, 37C
Raphanus sativus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4
-
Raphanus sativus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2
6
almost inactive below and above
Raphanus sativus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Raphanus sativus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
1 mM, almost complete inhibition
Raphanus sativus
iodoacetic acid
1 mM, about 80% inhibition
Raphanus sativus
additional information
not inhibitory: Mn2+, Fe3+, Cu2+, Mg2+, Ba2+, Ca2+, Co2+, Zn2+, Cd2+
Raphanus sativus
sodium dodecylsulfate
1 mM, about 80% inhibition
Raphanus sativus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
p-nitrophenyl-beta-galactoside
pH 4.0, 37C
Raphanus sativus
0.77
-
beta-(1-3)-D-galactotriose
pH 4.0, 37C
Raphanus sativus
1.77
-
beta-(1-3)-galactobiose
pH 4.0, 37C
Raphanus sativus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33654
-
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
44981
-
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme expressed in Pichia pastoris
Raphanus sativus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
64.9
-
pH 4.0, 37C
Raphanus sativus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-(1-3)-D-galactan + H2O
8% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-3)-D-galactan + D-galactose
-
-
-
?
beta-(1-3)-D-galactotriose + H2O
44% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-3)-D-galactobiose + D-galactose
-
-
-
?
beta-(1-3)-galactobiose + H2O
32% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
D-galactose
-
-
-
?
beta-(1-6)-galactopentaose + H2O
50% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactotetraose + D-galactose
-
-
-
?
beta-(1-6)-galactotetraose + H2O
68% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactotriose + D-galactose
-
-
-
?
beta-(1-6)-galactotriose + H2O
49% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-(1-6)-D-galactobiose + D-galactose
-
-
-
?
beta-galactosido-(1-3)-beta-galactosido-(1-6)-galactoside + H2O
42% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-galactosido-(1-3)-beta-galactoside + D-galactose
-
-
-
-
beta-galactosido-(1-6)-beta-galactosido-(1-3)-galactoside + H2O
40% of the activity with p-nitrophenyl-beta-galactoside
666634
Raphanus sativus
beta-galactosido-(1-6)-beta-galactoside + D-galactose
-
-
-
-
additional information
specific hydrolysis of beta-1,3 and beta-1,6 galactooligosaccharides
666634
Raphanus sativus
?
-
-
-
-
p-nitrophenyl-beta-galactoside + H2O
-
666634
Raphanus sativus
p-nitrophenol + beta-galactose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated
Raphanus sativus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Raphanus sativus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
65
-
loss of 90% of activity
Raphanus sativus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
28.6
-
beta-(1-3)-galactobiose
pH 4.0, 37C
Raphanus sativus
32.5
-
beta-(1-3)-D-galactotriose
pH 4.0, 37C
Raphanus sativus
123
-
p-nitrophenyl-beta-galactoside
pH 4.0, 37C
Raphanus sativus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4
-
Raphanus sativus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2
6
almost inactive below and above
Raphanus sativus
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
Degradative enzymes for type ...
Bifidobacterium longum subsp. Longum
Appl. Microbiol. Biotechnol.
103
1299-1310
2019
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
-
-
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
9
-
14
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
5
-
2
2
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
10
1
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
4
1
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-