Literature summary for 3.2.1.141 extracted from

  • Kato, M.; Miura, Y.; Kettoku, M.; Komeda, T.; Iwamatsu, A.; Kobayashi, K.
    Reaction mechanism of a new glycosyltrehalose-hydrolyzing enzyme isolated from the hyperthermophilic archaeum, Sulfolobus solfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 925-928.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.8
-
maltosyltriosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
4.7
-
maltosylpentaosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
5.6
-
maltosyltetraosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
22.4
-
maltosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q55088
-
-
Saccharolobus solfataricus KM1 Q55088
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltopentaose
-
?
maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltopentaose
-
?
maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotetraose
-
?
maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotetraose
-
?
maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltose
-
?
maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltose
-
?
maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotriose
-
?
maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotriose
-
?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus
70 85
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
6 h, stable Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Saccharolobus solfataricus
4.5 5.5
-
Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
4.8