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Literature summary for 3.2.1.14 extracted from

  • Mohamed, S.; Bouacem, K.; Mechri, S.; Addou, N.A.; Laribi-Habchi, H.; Fardeau, M.L.; Jaouadi, B.; Bouanane-Darenfed, A.; Hacene, H.
    Purification and biochemical characterization of a novel acido-halotolerant and thermostable endochitinase from Melghiribacillus thermohalophilus strain Nari2AT (2019), Carbohydr. Res., 473, 46-56 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Activating Compound

Activating Compound Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 110% activity at 2 mM Melghiribacillus thermohalophilus
chloroform about 160% activity at 25%(v/v) Melghiribacillus thermohalophilus
Cyclohexane about 148% activity at 25%(v/v) Melghiribacillus thermohalophilus
dimethylformamide about % activity at 25%(v/v) Melghiribacillus thermohalophilus
DMF about % activity at 25%(v/v) Melghiribacillus thermohalophilus
DMSO about 160% activity at 25%(v/v) Melghiribacillus thermohalophilus
Isopropanol about 150% activity at 25%(v/v) Melghiribacillus thermohalophilus
n-decane about 110% activity at 25%(v/v) Melghiribacillus thermohalophilus
n-hexane about 130% activity at 25%(v/v) Melghiribacillus thermohalophilus
NaCl maximum activity at 4 M Melghiribacillus thermohalophilus

General Stability

General Stability Organism
4 M NaCl. Beyond 4M NaCl, chitinase activity decreases progressively and retains 30% of its relative activity at 10 M NaCl Melghiribacillus thermohalophilus
more than 30% of residual activities are obtained at the range of 0.5-10 M NaCl with optimal activity reached at Melghiribacillus thermohalophilus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 20% residual activity at 10 mM Melghiribacillus thermohalophilus
5,5'-dithio-bis(2-nitrobenzoic acid) 2% residual activity at 10 mM Melghiribacillus thermohalophilus
acetonitrile complete inhibition at 25%(v/v) Melghiribacillus thermohalophilus
Ag+ 10% residual activity at 10 mM Melghiribacillus thermohalophilus
Al3+ 73% residual activity at 10 mM Melghiribacillus thermohalophilus
Ba2+ 96% residual activity at 10 mM Melghiribacillus thermohalophilus
Cd2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
Cu2+ 51% residual activity at 10 mM Melghiribacillus thermohalophilus
dithiothreitol 8% residual activity at 10 mM Melghiribacillus thermohalophilus
ethyl acetate complete inhibition at 25%(v/v) Melghiribacillus thermohalophilus
Fe2+ 32% residual activity at 10 mM Melghiribacillus thermohalophilus
Hg2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
isooctane about 90% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
additional information not inhibited by 2,4,6-trinitrobenzenesulfonic acid, phenylmethylsulfonyl fluoride, diethyldicarbonate, and N-bromosuccinimide Melghiribacillus thermohalophilus
N-Acetylimidazole 94% residual activity at 2 mM Melghiribacillus thermohalophilus
N-ethylmaleimide complete inhibition at 2 mM Melghiribacillus thermohalophilus
n-heptane about 80% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
n-hexadecane about 70% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
Ni2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
p-chloromercuribenzoic acid complete inhibition at 2 mM Melghiribacillus thermohalophilus
Toluene about 80% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
Zn2+ 56% residual activity at 10 mM Melghiribacillus thermohalophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 280% activity at 2 mM Melghiribacillus thermohalophilus
Mg2+ 175% activity at 2 mM Melghiribacillus thermohalophilus
Mn2+ 127% activity at 2 mM Melghiribacillus thermohalophilus
additional information not influenced by Co2+ Melghiribacillus thermohalophilus

Organism

Organism UniProt Comment Textmining
Melghiribacillus thermohalophilus
-
-
-
Melghiribacillus thermohalophilus Nari2AT
-
-
-

Purification (Commentary)

Purification (Comment) Organism
UNO S-1 column chromatography and Zorbax PSM 300 HPSEC column chromatography Melghiribacillus thermohalophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D- N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O 535% activity compared to colloidal chitin Melghiribacillus thermohalophilus 4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide
-
 ?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O 198% activity compared to colloidal chitin Melghiribacillus thermohalophilus 4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
 ?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O 150% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
beta-D-N,N',N''-triacetylchitotriose + H2O 86% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
chitin azure + H2O 97% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
chitin azure + H2O 97% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
chitooligosaccharide + H2O 77% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
colloidal chitin + H2O 100% activity Melghiribacillus thermohalophilus N-acetyl-beta-D-glucosamine + ?
-
 ?
colloidal chitin + H2O 100% activity Melghiribacillus thermohalophilus Nari2AT N-acetyl-beta-D-glucosamine + ?
-
 ?
glycol chitin + H2O 92% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
glycol chitin + H2O 92% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
glycol chitosan + H2O 88% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
glycol chitosan + H2O 88% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
additional information the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin Melghiribacillus thermohalophilus ?
-
 ?
additional information the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
xylan + H2O 40% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45201, calculated from amino acid sequence Melghiribacillus thermohalophilus
monomer 1 * 45000, SDS-PAGE Melghiribacillus thermohalophilus

Synonyms

Synonyms Comment Organism
ChiA-Mt45
-
 Melghiribacillus thermohalophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Melghiribacillus thermohalophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Melghiribacillus thermohalophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 6 the half-life times of the enzyme at pH 2.0, 3.0, 4.0, 5.0, and 6.0 are 18, 14, 10, 6, and 2 h, respectively Melghiribacillus thermohalophilus