Literature summary for 3.2.1.14 extracted from

Honda, S.; Wakita, S.; Sugahara, Y.; Kawakita, M.; Oyama, F.; Sakaguchi, M.
Characterization of two Listeria innocua chitinases of different sizes that were expressed in Escherichia coli (2016), Appl. Microbiol. Biotechnol., 100, 8031-8041 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Listeria innocua

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.127	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi78, at pH 6.0 and 50°C	Listeria innocua
0.885	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi35, at pH 5.0 and 50°C	Listeria innocua

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chitin + H2O	Listeria innocua	-	?	-	?
chitin + H2O	Listeria innocua ATCC 33090	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Listeria innocua	A0A146FAY1	-	-
Listeria innocua	A0A146FAZ1	-	-
Listeria innocua ATCC 33090	A0A146FAY1	-	-
Listeria innocua ATCC 33090	A0A146FAZ1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni Sepharose column chromatography	Listeria innocua

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O	-	Listeria innocua	4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose	-	?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O	-	Listeria innocua ATCC 33090	4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose	-	?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O	-	Listeria innocua	4-nitrophenol + beta-D-N,N'-diacetylchitobiose	-	?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O	-	Listeria innocua ATCC 33090	4-nitrophenol + beta-D-N,N'-diacetylchitobiose	-	?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O	-	Listeria innocua	beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose	-	?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O	-	Listeria innocua ATCC 33090	beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose	-	?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O	-	Listeria innocua	beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose	-	?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O	-	Listeria innocua ATCC 33090	beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose	-	?
chitin + H2O	-	Listeria innocua	?	-	?
chitin + H2O	-	Listeria innocua ATCC 33090	?	-	?
colloidal chitin + H2O	-	Listeria innocua	beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine	-	?
colloidal chitin + H2O	-	Listeria innocua ATCC 33090	beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine	-	?
colloidal chitin + H2O	-	Listeria innocua	N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine	-	?
colloidal chitin + H2O	-	Listeria innocua ATCC 33090	N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine	-	?
additional information	isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose	Listeria innocua	?	-	?
additional information	isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose	Listeria innocua ATCC 33090	?	-	?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O	-	Listeria innocua	N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose	-	?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O	-	Listeria innocua ATCC 33090	N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose	-	?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O	-	Listeria innocua	N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose	-	?
tri-N-acetyl-chitotriose + H2O	substrate for isoform Chi78	Listeria innocua	beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine	-	?

Subunits

Subunits	Comment	Organism
?	x * 38000, isoform Chi35, SDS-PAGE	Listeria innocua
?	x * 82000, isoform Chi78, SDS-PAGE	Listeria innocua

Synonyms

Synonyms	Comment	Organism
Chi35	isoform	Listeria innocua
Chi78	isoform	Listeria innocua

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Listeria innocua

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	60	the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi35 has a melting temperature of 60.8°C	Listeria innocua
40	60	the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi78 is almost completely inactivated in 60 min at 60°C and shows a melting temperature of 51.5°C	Listeria innocua

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.4	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi78, at pH 6.0 and 50°C	Listeria innocua
51.1	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi35, at pH 5.0 and 50°C	Listeria innocua

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Listeria innocua
6	-	-	Listeria innocua

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3.5	-	isoform Chi35 retains full activity at pH 3.5 for 24 h	Listeria innocua
3.5	-	the activity of isoform Chi78 is decreased to approximately 20% in 24 h at pH 3.5 at 4 °C	Listeria innocua

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
60	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi35, at pH 5.0 and 50°C	Listeria innocua
80	-	4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside	isoform Chi78, at pH 6.0 and 50°C	Listeria innocua