Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli strain JM109, removal of the C-terminal VTS of 24 bp and replacement of the N-terminal signal sequence of yam chitinase with alpha-factor of the expression vector pPICZalphaA, recombinant expression in Pichia pastoris strain X-33 an secretion to the culture medium | Dioscorea polystachya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.233 mg/ml at pH 8.0, 32°C, substrate glycol chitin, recombinant enzyme | Dioscorea polystachya |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Dioscorea polystachya | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant deglycosylated enzyme, SDS-PAGE | Dioscorea polystachya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Dioscorea polystachya | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dioscorea polystachya | Q852P9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation by endoglycosidase H | Dioscorea polystachya |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme 10fold from Pichia pastoris culture supernatant by several different steps of anion exchange chromatography and gel filtration | Dioscorea polystachya |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.23 | - |
purified recombinant enzyme, pH 8.0, 32°C, substrate glycolchitin | Dioscorea polystachya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Dioscorea polystachya | ? | - |
? | |
glycol chitin + H2O | - |
Dioscorea polystachya | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant deglycosylated enzyme, SDS-PAGE | Dioscorea polystachya |
Synonyms | Comment | Organism |
---|---|---|
class IV chitinase | - |
Dioscorea polystachya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Dioscorea polystachya |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified native deglycosylated enzyme, pH 8.0, 15 min, completely stable | Dioscorea polystachya |
60 | - |
purified native deglycosylated enzyme, pH 8.0, 15 min, over 90% activity remaining | Dioscorea polystachya |
70 | - |
purified native deglycosylated enzyme, pH 8.0, 15 min, over 60% activity remaining | Dioscorea polystachya |
75 | - |
purified native deglycosylated enzyme, pH 8.0, 15 min, 10% activity remaining | Dioscorea polystachya |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
glycol chitin | pH 8.0, 32°C, recombinant enzyme | Dioscorea polystachya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
main larger peak | Dioscorea polystachya |
8 | - |
second smaller peak | Dioscorea polystachya |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | activity range, profile overview | Dioscorea polystachya |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | purified native deglycosylated enzyme, 32°C, completely stable | Dioscorea polystachya |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family GH 19 | Dioscorea polystachya |
physiological function | recombinant Pichia pastoris-produced chitinase shows strong lytic activity against Fusarium oxysporum and Phytophthora nicotianae | Dioscorea polystachya |