Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Stenotrophomonas maltophilia | |
Cu2+ | - |
Stenotrophomonas maltophilia | |
Hg2+ | - |
Stenotrophomonas maltophilia | |
Zn2+ | - |
Stenotrophomonas maltophilia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Stenotrophomonas maltophilia | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the enzyme | Stenotrophomonas maltophilia | |
Mg2+ | stabilizes the enzyme | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
isolated from the rhizosphere of winter wheat | - |
Stenotrophomonas maltophilia MUJ | - |
isolated from the rhizosphere of winter wheat | - |
Purification (Comment) | Organism |
---|---|
native enzyme 5.8fold from culture supernatant by ammonium sulfate fractionation, ultrafiltration, and chitin affinity chromatography | Stenotrophomonas maltophilia |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.3 | - |
purified enzyme, pH 6.8, 45°C | Stenotrophomonas maltophilia |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Stenotrophomonas maltophilia |
More | enzyme peptide mapping, overview | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
chitinolytic enzyme | - |
Stenotrophomonas maltophilia |
More | the enzyme belongs to family 18 of glycosyl hydrolases | Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Stenotrophomonas maltophilia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | activity range, 80% of maximal activity at 40°C and 50°C, inactive at 60°C | Stenotrophomonas maltophilia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, 3 h, completely stable | Stenotrophomonas maltophilia |
45 | - |
purified enzyme, 3 h, loss of 15% activity | Stenotrophomonas maltophilia |
50 | - |
purified enzyme, 2 h, loss of 70% activity | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Stenotrophomonas maltophilia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | activity range, 10% of maximal activity at pH 5.5 and pH 8.5 | Stenotrophomonas maltophilia |
General Information | Comment | Organism |
---|---|---|
physiological function | the purified enzyme inhibits the growth of fungal phytopathogens belonging to the genera Fusarium, Rhizoctonia and Alternaria | Stenotrophomonas maltophilia |