Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
S33W | strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
W231A | strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayed reduced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
W70A | strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant enzyme shows severe loss in hydrolytic activities. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
Y245W | strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayes enhanced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.163 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231F | Vibrio harveyi | |
0.171 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33A | Vibrio harveyi | |
0.185 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W70A | Vibrio harveyi | |
0.189 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231A | Vibrio harveyi | |
0.201 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme Y245W | Vibrio harveyi | |
0.21 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33W | Vibrio harveyi | |
0.218 | - |
chitohexaose | pH 5.5, 37°C, wild-type enzyme | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | low activity with crystallin chitin, high activity with colloidal chitin | Vibrio harveyi | ? | - |
? | |
chitohexaose + H2O | - |
Vibrio harveyi | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | - |
Vibrio harveyi | p-nitrophenol + N,N'-diacetylchitobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chitinase A | - |
Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W70A | Vibrio harveyi | |
0.6 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W231A | Vibrio harveyi | |
0.9 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym S33A | Vibrio harveyi | |
0.9 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W231F | Vibrio harveyi | |
1.2 | - |
chitin | pH 5.5, 37°C, colloidal chitin, wild-type enzyme | Vibrio harveyi | |
1.4 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym S33W | Vibrio harveyi | |
1.7 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym Y245W | Vibrio harveyi | |
2.4 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231F | Vibrio harveyi | |
2.5 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W70A | Vibrio harveyi | |
2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33A | Vibrio harveyi | |
2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231A | Vibrio harveyi | |
2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme Y245W | Vibrio harveyi | |
2.8 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33W | Vibrio harveyi | |
2.9 | - |
chitohexaose | pH 5.5, 37°C, wild-type enzyme | Vibrio harveyi |