Application | Comment | Organism |
---|---|---|
industry | the enzyme has a great potential for industrial applications due to its high yield and broad pH stability | Renibacterium sp. QD1 |
Cloned (Comment) | Organism |
---|---|
- |
Renibacterium sp. QD1 |
Protein Variants | Comment | Organism |
---|---|---|
E160A | mutant enzyme with decreased thermal stability and reduced specific activity | Renibacterium sp. QD1 |
E160Q | mutant enzyme with decreased thermal stability and reduced specific activity | Renibacterium sp. QD1 |
K163A | mutant enzyme with decreased thermal stability and reduced specific activity | Renibacterium sp. QD1 |
T114A | mutant enzyme with decreased thermal stability and slightly reduced specific activity | Renibacterium sp. QD1 |
General Stability | Organism |
---|---|
the hydrogen-bond network around Glu160 plays important roles in stabilization of the enzyme | Renibacterium sp. QD1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
SDS-PAGE | Renibacterium sp. QD1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Renibacterium sp. QD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Renibacterium sp. QD1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
250.87 | - |
pH 5.6, 55°C, mutant enzyme K163A | Renibacterium sp. QD1 |
261.73 | - |
pH 5.6, 55°C, mutant enzyme E160A | Renibacterium sp. QD1 |
283.93 | - |
pH 5.6, 55°C, mutant enzyme E160Q | Renibacterium sp. QD1 |
328.05 | - |
pH 5.6, 55°C, mutant enzyme T114A | Renibacterium sp. QD1 |
335.07 | - |
pH 5.6, 55°C, wild-type enzyme | Renibacterium sp. QD1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | - |
Renibacterium sp. QD1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Renibacterium sp. QD1 |
Synonyms | Comment | Organism |
---|---|---|
chitosanase CsnA | - |
Renibacterium sp. QD1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Renibacterium sp. QD1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35.55 | - |
Tm-value of mutant enzyme E160A | Renibacterium sp. QD1 |
36.02 | - |
Tm-value of mutant enzyme E160Q | Renibacterium sp. QD1 |
37.36 | - |
Tm-value of mutant enzyme T114A | Renibacterium sp. QD1 |
38.86 | - |
Tm-value of mutant enzyme K163A | Renibacterium sp. QD1 |
40.36 | - |
Tm-value of wild-type enzyme | Renibacterium sp. QD1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Renibacterium sp. QD1 |