Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | isoform A shows 111% activity at 2.5 mM | Bambusa oldhamii | |
p-hydroxymercuribenzoate | isoform A shows 112% activity at 0.5 mM | Bambusa oldhamii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dinitro-1-fluorobenzene | isoform A shows 95% residual activity at 0.625 mM, isoform B shows 84% residual activity at 0.625 mM | Bambusa oldhamii | |
Ag+ | isoform A shows 91% residual activity at 1 mM, isoform B shows 86% residual activity at 1 mM | Bambusa oldhamii | |
ethyl acetimidate | isoform B shows 56% residual activity at 250 mM | Bambusa oldhamii | |
Hg2+ | isoform B shows 77% residual activity at 0.25 mM | Bambusa oldhamii | |
additional information | not inhibited by diethyl dicarbonate, 1,2-cyclohexanedione, and p-hydroxymercuribenzoate | Bambusa oldhamii | |
N-Acetylimidazole | isoform A shows 96% residual activity at 5 mM, isoform B shows 84% residual activity at 5 mM | Bambusa oldhamii | |
N-bromosuccinimide | isoform A shows 12% residual activity at 2.5 mM, isoform B is completely inhibited at 2.5 mM | Bambusa oldhamii | |
phenylmethylsulfonyl fluoride | isoform B shows 86% residual activity at 2.5 mM | Bambusa oldhamii | |
Woodward's reagent K | N-ethyl-5-phenylisoazoline-3'-sulfonate, isoform A shows 95% residual activity at 50 mM, isoform B shows 1% residual activity at 50 mM | Bambusa oldhamii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoform B shows 117% residual activity at 1 mM | Bambusa oldhamii | |
additional information | not influenced by Cu2+, Mg2+, K+, and EDTA | Bambusa oldhamii | |
Na+ | isoform A shows 111% activity at 5 mM | Bambusa oldhamii | |
Zn2+ | isoform B shows 111% residual activity at 1 mM | Bambusa oldhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16400 | - |
x * 16400, isoform B, SDS-PAGE | Bambusa oldhamii |
16400 | - |
x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE | Bambusa oldhamii |
24500 | - |
x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE | Bambusa oldhamii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Bambusa oldhamii | chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B | D-glucosamine + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bambusa oldhamii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sephacryl S-100 gel filtration | Bambusa oldhamii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Bambusa oldhamii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0065 | - |
crude extract, at pH and °C | Bambusa oldhamii |
0.243 | - |
isoform B, after 115.1fold purification, at pH 4.5 and 50°C | Bambusa oldhamii |
0.753 | - |
isoform A, after 115.1fold purification, at pH 4.5 and 50°C | Bambusa oldhamii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl chitin + H2O | isoform A shows 41.8% activity and isoform B 17.2% activity compared to chitosan | Bambusa oldhamii | ? | - |
? | |
chitin + H2O | isoform A shows 7.8% activity and isoform B 10.8% activity compared to chitosan | Bambusa oldhamii | ? | - |
? | |
chitosan + H2O | chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B | Bambusa oldhamii | D-glucosamine + ? | - |
? | |
glycol chitin + H2O | isoform A shows 29.7% activity and isoform B 14.1% activity compared to chitosan | Bambusa oldhamii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16400, isoform B, SDS-PAGE | Bambusa oldhamii |
? | x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE | Bambusa oldhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
isoform B | Bambusa oldhamii |
70 | - |
isoform A | Bambusa oldhamii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | isoform A is stable between 30 and 80°C, whereas isoform B is stable between 30 and 50°C after 60 min incubation | Bambusa oldhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Bambusa oldhamii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | both purified isoforms A and B retain approximately 70-100% relative activity between pH 3.0 and 6.0 but rapidly lose activity above pH 6.0 | Bambusa oldhamii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bambusa oldhamii | isoform A, isoelectric focusing | - |
4.3 |
Bambusa oldhamii | isoform B, isoelectric focusing | - |
9.2 |