Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 2% nonionic surfactant of Triton X-100 activates by 51% at 25°C for 30 min | Pseudomonas sp. | |
Tween 20 | 2% nonionic surfactant of Tween 20 activates by 38% at 25°C for 30 min | Pseudomonas sp. | |
Tween 40 | 2% nonionic surfactant of Tween 40 activates by 6% at 25°C for 30 min | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
EDTA | 10 mM inhibits by 86% at 25°C for 30 min | Pseudomonas sp. | |
Fe2+ | 5 mM inhibits by 67% at 25°C for 30 min | Pseudomonas sp. | |
Mn2+ | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
PMSF | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
SDS | 2 mM of the anionic surfactant SDS completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
Triton X-100 | 0.5% nonionic surfactant of Triton X-100 inhibits by 56% at 25°C for 30 min | Pseudomonas sp. | |
Tween 20 | 0.5% nonionic surfactant of Tween 20 inhibits by 16% at 25°C for 30 min | Pseudomonas sp. | |
Tween 40 | 0.5% nonionic surfactant of Tween 40 inhibits by 29% at 25°C for 30 min | Pseudomonas sp. | |
Zn2+ | 5 mM inhibits by 21% at 25°C for 30 min | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM activates by 11% at 25°C for 30 min | Pseudomonas sp. | |
additional information | 5 mM of Mg2+, Ba2+ have no effect on activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDSPAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. TKU015 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, to homogeneity, 6.4fold | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
culture supernatant | Pseudomonas sp. |
59.1 | - |
6.4fold purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan | Pseudomonas sp. | ? | - |
? | |
chitosan + H2O | activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan | Pseudomonas sp. TKU015 | ? | - |
? | |
additional information | no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan | Pseudomonas sp. | ? | - |
? | |
additional information | no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan | Pseudomonas sp. TKU015 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDSPAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Chs1 | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | maintains its initial activity, has almost half of its activity at 50°C but is completely inactivated at 70°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Pseudomonas sp. |