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Literature summary for 3.2.1.131 extracted from

  • Wang, L.; Shi, H.; Xu, B.; Li, X.; Zhang, Y.; Wang, F.
    Characterization of Thermotoga thermarum DSM 5069 alpha-glucuronidase and synergistic degradation of xylan (2016), BioResources, 11, 5767-5779 .
No PubMed abstract available
Search for more literature for 3.2.1.131

Activating Compound

Activating Compound Comment Organism Structure
EDTA about 115% activity at 1 mM Pseudothermotoga thermarum
Triton X-100 about 105% activity at 1 mM Pseudothermotoga thermarum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Pseudothermotoga thermarum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ about 60% residual activity at 1 mM Pseudothermotoga thermarum
Cu2+ about 20% residual activity at 1 mM Pseudothermotoga thermarum
Fe2+ about 60% residual activity at 1 mM Pseudothermotoga thermarum
Ni2+ about 70% residual activity at 1 mM Pseudothermotoga thermarum
SDS complete inhibition at 1 mM Pseudothermotoga thermarum
Zn2+ about 10% residual activity at 1 mM Pseudothermotoga thermarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.02
-
 aldouronic acids at pH 6.0 and 80°C Pseudothermotoga thermarum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudothermotoga thermarum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 120% activity at 1 mM Pseudothermotoga thermarum
additional information not influenced by Ca2+, Mg2+, and Mn2+ Pseudothermotoga thermarum

Organism

Organism UniProt Comment Textmining
Pseudothermotoga thermarum F7YUS1
-
-
Pseudothermotoga thermarum DSM 5069 F7YUS1
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Pseudothermotoga thermarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-glucuronic acid + H2O
-
 Pseudothermotoga thermarum ?
-
 ?
4-O-methyl-glucuronic acid + H2O
-
 Pseudothermotoga thermarum DSM 5069 ?
-
 ?
aldouronic acids + H2O
-
 Pseudothermotoga thermarum alpha-1,2-linked alpha-D-glucuronic acid + ?
-
 ?
aldouronic acids + H2O
-
 Pseudothermotoga thermarum DSM 5069 alpha-1,2-linked alpha-D-glucuronic acid + ?
-
 ?
additional information no activity towards debranched arabinan, 1,4-beta-D-mannan, galactan, 4-nitrophenyl xylopyranoside, or 4-nitrophenyl alpha-L-arabinofuranoside Pseudothermotoga thermarum ?
-
 ?
additional information no activity towards debranched arabinan, 1,4-beta-D-mannan, galactan, 4-nitrophenyl xylopyranoside, or 4-nitrophenyl alpha-L-arabinofuranoside Pseudothermotoga thermarum DSM 5069 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, His-tagged enzyme, SDS-PAGE Pseudothermotoga thermarum

Synonyms

Synonyms Comment Organism
AguA
-
 Pseudothermotoga thermarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Pseudothermotoga thermarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the enzyme maintains 98%, 55% and 15% activity after incubation at 65°C, 70°C, or 75°C, respectively, for 2 h Pseudothermotoga thermarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
117
-
 aldouronic acids at pH 6.0 and 80°C Pseudothermotoga thermarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudothermotoga thermarum