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Literature summary for 3.2.1.127 extracted from

  • Megson, Z.A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.B.; Stafford, G.P.; Messner, P.; Schaeffer, C.
    Characterization of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia (2015), Virulence, 6, 282-292 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.127

Inhibitors

Inhibitors Comment Organism Structure
deoxyfuconojirimycin competitive Tannerella forsythia
fucose competitive Tannerella forsythia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 4-nitrophenyl alpha-L-fucopyranoside 22°C, pH 9.0 Tannerella forsythia

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information following knock-out of the Tffuc1 gene and analyzing biofilm formation and cell invasion/adhesion of the mutant in comparison to the wild-type, it is most likely that the enzyme does not act extracellularly Tannerella forsythia
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
-
 Tannerella forsythia

Organism

Organism UniProt Comment Textmining
Tannerella forsythia G8UMQ6
-
-
Tannerella forsythia ATCC 43037 G8UMQ6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyl-N-acetyllactosamine + H2O
-
 Tannerella forsythia alpha-L-fucose + ?
-
 ?
2'-fucosyl-N-acetyllactosamine + H2O
-
 Tannerella forsythia ATCC 43037 alpha-L-fucose + ?
-
 ?
2-fucosyllactose + H2O
-
 Tannerella forsythia alpha-L-fucose + lactose
-
 ?
2-fucosyllactose + H2O
-
 Tannerella forsythia ATCC 43037 alpha-L-fucose + lactose
-
 ?
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + alpha-L-fucopyranose
-
 ?
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
-
 Tannerella forsythia alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
additional information the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal Tannerella forsythia ?
-
 ?
additional information the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal Tannerella forsythia ATCC 43037 ?
-
 ?

Synonyms

Synonyms Comment Organism
bfo_2737
-
 Tannerella forsythia
TfFuc1
-
 Tannerella forsythia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Tannerella forsythia

pH Range

pH Minimum pH Maximum Comment Organism
8 10.2 pH 8.0: about 70% of maximal activity, pH 10.2: about 40% of maximal activity Tannerella forsythia

General Information

General Information Comment Organism
physiological function the biological role of the enzyme in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism Tannerella forsythia