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Literature summary for 3.2.1.123 extracted from

  • Ito, M.; Ikegami, Y.; Yamagata, T.
    Kinetics of endoglycoceramidase action toward cell-surface glycosphingolipids of erythrocytes (1993), Eur. J. Biochem., 218, 645-649.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
Activator protein increases the activity of EGCase II 468fold to 616fold Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide from horse erythrocyte cell surface, pH 7, 37°C Rhodococcus sp.
0.047
-
 GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide i.e. Gg3Cer from guinea pig erythrocyte cell surface, pH 7, 37°C Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 molecular species: II
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. Gg3Cer Rhodococcus sp. GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
 ?
glycosphingolipid + H2O cell-surface glycosphingolipids of erythrocytes, horse-erythrocyte cell-surface GM3 and guinea pig erythrocyte cell-surface Gg3Cer Rhodococcus sp. ?
-
 ?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
7
-
 90% activity for NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide, 64% activity for Gg3Cer Rhodococcus sp.