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Literature summary for 3.2.1.116 extracted from

  • Lee, Y.; Park, D.; Choi, Y.
    Characterization of maltotriose production by hydrolyzing of soluble starch with alpha-amylase from Microbulbifer thermotolerans DAU221 (2015), Appl. Microbiol. Biotechnol., 99, 3901-3911 .
No PubMed abstract available
Search for more literature for 3.2.1.116

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli using the pCold I vector Microbulbifer thermotolerans

General Stability

General Stability Organism
the enzyme retains significant activity (50%) in the presence up to 2 M NaCl Microbulbifer thermotolerans

Inhibitors

Inhibitors Comment Organism Structure
acetone 20% (v/v), 2 h, 20°C, 12% loss of activity Microbulbifer thermotolerans
Benzene 20% (v/v), 2 h, 20°C, 7% loss of activity Microbulbifer thermotolerans
chloroform 20% (v/v), 2 h, 20°C, 71% loss of activity Microbulbifer thermotolerans
Co2+ 10 mM, 47% loss of activity Microbulbifer thermotolerans
Cu2+ 10 mM, 81% loss of activity Microbulbifer thermotolerans
EDTA 10 mM, 83% loss of activity Microbulbifer thermotolerans
ethanol 20% (v/v), 2 h, 20°C, 39% loss of activity Microbulbifer thermotolerans
Fe3+ 10 mM, 81% loss of activity Microbulbifer thermotolerans
Hg2+ 10 mM, 83% loss of activity Microbulbifer thermotolerans
methanol 20% (v/v), 2 h, 20°C, 44% loss of activity Microbulbifer thermotolerans
n-butanol 20% (v/v), 2 h, 20°C, 49% loss of activity Microbulbifer thermotolerans
Ni2+ 10 mM, 23% loss of activity Microbulbifer thermotolerans
Zn2+ 10 mM, 82% loss of activity Microbulbifer thermotolerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 soluble starch KM-value: 1.08 mg/ml Microbulbifer thermotolerans

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl high concentrations of NaCl (0.5, 1, 1.5, 2, 3, and 4 M) slightly decreases the activity Microbulbifer thermotolerans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 20% (v/v), 2 h, 20°C, 12% loss of activity Microbulbifer thermotolerans
benzene 20% (v/v), 2 h, 20°C, 7% loss of activity Microbulbifer thermotolerans
chloroform 20% (v/v), 2 h, 20°C, 71% loss of activity Microbulbifer thermotolerans
Ethanol 20% (v/v), 2 h, 20°C, 39% loss of activity Microbulbifer thermotolerans
hexane the enzyme exhibits extreme stability toward hexane. 1% loss of activity after 2 h at 20°C in 20% (v/v) hexane Microbulbifer thermotolerans
Methanol 20% (v/v), 2 h, 20°C, 44% loss of activity Microbulbifer thermotolerans
n-Butanol 20% (v/v), 2 h, 20°C, 49% loss of activity Microbulbifer thermotolerans

Organism

Organism UniProt Comment Textmining
Microbulbifer thermotolerans A0A0A0Q4S7
-
-
Microbulbifer thermotolerans DAU221 A0A0A0Q4S7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbulbifer thermotolerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
 Microbulbifer thermotolerans maltotriose + ?
-
 ?
soluble starch + H2O
-
 Microbulbifer thermotolerans DAU221 maltotriose + ?
-
 ?

Synonyms

Synonyms Comment Organism
AmyA
-
 Microbulbifer thermotolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in sodium phosphate buffer (pH 6.0) Microbulbifer thermotolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 above 90 % activity until 6 h and 80 % activity until 12 h Microbulbifer thermotolerans
40
-
 the preincubated enzyme maintains above 90% activity until 2 h, 80% activity until 4 h, 70% activity until 8 h, and 60% activity until 12 h Microbulbifer thermotolerans
50
-
 the preincubated enzyme maintains 50% activity until 1 h, 30% activity until 6 h, and 20% activity until 12 h Microbulbifer thermotolerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.35
-
-
 Microbulbifer thermotolerans