Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | poor effect of 2-mercaptoethanol | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Bacillus subtilis | |
Ca2+ | - |
Bacillus subtilis | |
EDTA | - |
Bacillus subtilis | |
H2O2 | - |
Bacillus subtilis | |
Mn2+ | - |
Bacillus subtilis | |
Na+ | - |
Bacillus subtilis | |
SDS | - |
Bacillus subtilis | |
Triton X-100 | - |
Bacillus subtilis | |
Urea | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activates | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
205000 | - |
native PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus subtilis | - |
D-glucose + maltose + maltotriose | - |
? | |
starch + H2O | Bacillus subtilis N8 | - |
D-glucose + maltose + maltotriose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isolated from soil samples | - |
Bacillus subtilis N8 | - |
isolated from soil samples | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | amylase synthesis in strain N8 optimally occurs at 15°C and pH 10.0 on agar plates containing starch. Optimization of growth conditions, overview | Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Bacillus subtilis | D-glucose + maltose + maltotriose | - |
? | |
starch + H2O | - |
Bacillus subtilis N8 | D-glucose + maltose + maltotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 205000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
cold-active alpha-amylase | - |
Bacillus subtilis |
endo-1,4-alpha-D-glucanohydrolase | - |
Bacillus subtilis |
N8 alpha-amylase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | activity range | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
56% remaining activity after 24 h at pH 8.0, 50% after 24 h at pH 11.0-12.0 | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
56% remaining activity after 24 h at 25°C | Bacillus subtilis |
11 | 12 | 50% remaining activity after 24 h at 25°C | Bacillus subtilis |