Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged enzyme | Zunongwangia profunda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Vibrio sp. | |
Al3+ | - |
Pseudomonas sp. K6-28-040 | |
Al3+ | - |
Wangia sp. C52 | |
cetyltrimethylammonium bromide | CTAB | Zunongwangia profunda | |
Co2+ | - |
Streptomyces coelicolor A3 | |
Co2+ | - |
Zunongwangia profunda | |
Cu2+ | - |
Pseudomonas sp. K6-28-040 | |
Cu2+ | - |
Streptomyces coelicolor A3 | |
Cu2+ | - |
Vibrio sp. | |
Cu2+ | - |
Wangia sp. C52 | |
Cu2+ | - |
Zunongwangia profunda | |
DMSO | - |
Pseudomonas sp. K6-28-040 | |
EDTA | - |
Bacillus cereus | |
EDTA | - |
Pseudomonas sp. K6-28-040 | |
EDTA | - |
Rossellomorea aquimaris | |
EDTA | - |
Streptomyces sp. D1 | |
EDTA | - |
Vibrio sp. | |
EDTA | - |
Wangia sp. C52 | |
EDTA | - |
Zunongwangia profunda | |
EGTA | - |
Pseudomonas sp. K6-28-040 | |
EGTA | - |
Vibrio sp. | |
EGTA | - |
Wangia sp. C52 | |
Fe2+ | - |
Anoxybacillus beppuensis | |
Fe3+ | - |
Pseudomonas sp. K6-28-040 | |
Fe3+ | - |
Streptomyces sp. D1 | |
Fe3+ | - |
Wangia sp. C52 | |
Fe3+ | - |
Zunongwangia profunda | |
Hg2+ | - |
Anoxybacillus flavithermus | |
Hg2+ | - |
Bacillus cereus | |
Hg2+ | - |
Pseudomonas sp. K6-28-040 | |
Hg2+ | - |
Streptomyces coelicolor A3 | |
Hg2+ | - |
Streptomyces sp. D1 | |
Hg2+ | - |
Vibrio sp. | |
Hg2+ | - |
Wangia sp. C52 | |
Mg2+ | - |
Anoxybacillus beppuensis | |
Mn2+ | - |
Wangia sp. C52 | |
Mn2+ | - |
Zunongwangia profunda | |
additional information | the enzyme is resistant to SDS | Bacillus sp. TS-23 | |
Na+ | - |
Anoxybacillus beppuensis | |
NaCl | inhibits over 80% | Nocardiopsis sp. B2 | |
Ni2+ | - |
Zunongwangia profunda | |
Pb2+ | - |
Bacillus cereus | |
SDS | - |
Pseudomonas sp. K6-28-040 | |
SDS | - |
Wangia sp. C52 | |
SDS | - |
Zunongwangia profunda | |
Urea | - |
Streptomyces coelicolor A3 | |
Zn2+ | - |
Wangia sp. C52 | |
Zn2+ | - |
Zunongwangia profunda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km is 0.5 mg/ml and Vmax is 3.571 mmol/ml/mg | Anoxybacillus beppuensis | |
additional information | - |
additional information | Km is 1.7 mg/ml and Vmax is 0.5 mg/ml/min | Vibrio sp. | |
additional information | - |
additional information | Km is 1.73 mg/ml and Vmax is 1.24 mg/ml/min | Pseudomonas sp. K6-28-040 | |
additional information | - |
additional information | Km is 11.71 mg/ml and Vmax is 0.14 mg/ml/min | Zunongwangia profunda | |
additional information | - |
additional information | Km is 2.08 mg/ml and Vmax is 1.26 mg/ml/min | Wangia sp. C52 | |
additional information | - |
additional information | Km is 4.79 mg/ml and Vmax is 0.322 mM/min | Bacillus sp. ALSHL3 | |
additional information | - |
additional information | Km is 5.8 mg/ml and Vmax is 0.25 mg/ml/min | Aureobasidium pullulans | |
0.005 | - |
starch | pH 7.0, 55°C | Anoxybacillus flavithermus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Bacillus cereus | |
additional information | calcium ions show neither inhibitory nor potentiating activity on alpha-amylase activity, alpha-amylase extracted from marine Streptomyces strain A3 is mainly calcium independent | Streptomyces coelicolor A3 | |
NaCl | the enzyme is resistant against 4.5% NaCl | Halobacillus sp. amylus | |
NaCl | the enzyme is resistant to 11% NaCl | Bacillus sp. TSCVKK | |
NaCl | the enzyme is resistant to 4.5% NaCl | Bacillus sp. marini | |
NaCl | the enzyme is stable against 0.5-4.0 M, a salt-tolerant alpha-amylase | Zunongwangia profunda | |
NaCl | the enzyme is stable against 1.5 M NaCl | Bacillus sp. ALSHL3 | |
NaCl | the enzyme is stable against 10-17% | Streptomyces coelicolor A3 | |
NaCl | the enzyme is stable against 11-17% | Saccharopolyspora sp. A9 | |
NaCl | the enzyme is stable against 12% | Streptomyces sp. D1 | |
NaCl | the enzyme is stable against 3.5 M NaCl | Rossellomorea aquimaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anoxybacillus beppuensis | - |
- |
- |
Anoxybacillus beppuensis TSSC-1 | - |
- |
- |
Anoxybacillus flavithermus | - |
- |
- |
Aureobasidium pullulans | - |
- |
- |
Aureobasidium pullulans N13d | - |
- |
- |
Bacillus cereus | - |
- |
- |
Bacillus licheniformis | A1YR25 | - |
- |
Bacillus licheniformis NH1 | A1YR25 | - |
- |
Bacillus sp. A-3-15 | - |
- |
- |
Bacillus sp. ALSHL3 | - |
- |
- |
Bacillus sp. Ferdowsicous | P86331 | - |
- |
Bacillus sp. GM8901 | - |
- |
- |
Bacillus sp. I-3 | - |
- |
- |
Bacillus sp. KSM-K38 | Q93I48 | - |
- |
Bacillus sp. marini | - |
- |
- |
Bacillus sp. TS-23 | - |
- |
- |
Bacillus sp. TSCVKK | - |
- |
- |
Bacillus sp. WN 11 | - |
- |
- |
Bacillus sp. YX-1 | A9YDD9 | - |
- |
Bacillus subtilis | - |
- |
- |
Bacillus subtilis AX20 | - |
- |
- |
Bacillus subtilis DM-03 | - |
- |
- |
Chromohalobacter sp. TVSP 101 | - |
- |
- |
Chryseobacterium taeanense | A0A1G8DI07 | - |
- |
Chryseobacterium taeanense TKU001 | A0A1G8DI07 | - |
- |
Eisenia fetida | - |
- |
- |
Halobacillus sp. amylus | - |
- |
- |
Halobacillus sp. amylus HM454199 | - |
- |
- |
Marinobacter sp. EMB8 | - |
- |
- |
Nesterenkonia sp. | A0A2U8ZSD8 | - |
- |
Nesterenkonia sp. F | A0A2U8ZSD8 | - |
- |
Nocardiopsis sp. B2 | - |
- |
- |
Parageobacillus caldoxylosilyticus | C0LZ63 | i.e. Geobacillus caldoxylosilyticus | - |
Parageobacillus caldoxylosilyticus TK4 | C0LZ63 | i.e. Geobacillus caldoxylosilyticus | - |
Pseudomonas sp. K6-28-040 | - |
- |
- |
Rossellomorea aquimaris | - |
- |
- |
Rossellomorea aquimaris VITP4 | - |
- |
- |
Saccharopolyspora sp. A9 | - |
- |
- |
Streptomyces coelicolor A3 | Q9L035 | - |
- |
Streptomyces coelicolor A3 A3(2) | Q9L035 | - |
- |
Streptomyces coelicolor A3 ATCC BAA-471 | Q9L035 | - |
- |
Streptomyces coelicolor A3 M145 | Q9L035 | - |
- |
Streptomyces sp. D1 | - |
- |
- |
Sutcliffiella cohnii | A0A223KY92 | - |
- |
Sutcliffiella cohnii US147 | A0A223KY92 | - |
- |
Thermomonospora curvata | - |
- |
- |
Thermus sp. | - |
- |
- |
Vibrio sp. | - |
- |
- |
Wangia sp. C52 | - |
- |
- |
Zunongwangia profunda | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Vibrio sp. |
Purification (Comment) | Organism |
---|---|
163fold by starch affinity chromatography | Vibrio sp. |
16fold by ammonium sulfate fractionation and anion exchange chromatography | Bacillus cereus |
20fold by ammonium sulfate fractionation and hydrophobic interaction chromatography | Anoxybacillus beppuensis |
24fold by ammonium sulfate fractionation and gel filtration | Wangia sp. C52 |
39fold by ammonium sulfate fractionation and gel filtration | Saccharopolyspora sp. A9 |
44fold by gel filtration | Streptomyces coelicolor A3 |
44fold by gel filtration | Nocardiopsis sp. B2 |
5fold by ammonium sulfate fractionation and gel filtration | Pseudomonas sp. K6-28-040 |
GST-tagged enzyme 333fold by glutathione affinity chromatography | Zunongwangia profunda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | grows on maltose | Streptomyces coelicolor A3 | - |
additional information | grows on soluble starch | Bacillus cereus | - |
additional information | grows on starch | Anoxybacillus flavithermus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44 | - |
purified enzyme, pH and temperature not specified in the publication | Bacillus cereus |
135 | - |
purified enzyme, pH and temperature not specified in the publication | Pseudomonas sp. K6-28-040 |
146 | - |
purified enzyme, pH and temperature not specified in the publication | Wangia sp. C52 |
327 | - |
purified enzyme, pH 6.5, 60°C | Vibrio sp. |
1633 | - |
purified enzyme, pH and temperature not specified in the publication | Zunongwangia profunda |
1641 | - |
purified enzyme, pH and temperature not specified in the publication | Saccharopolyspora sp. A9 |
1755 | - |
purified enzyme, pH and temperature not specified in the publication | Streptomyces coelicolor A3 |
1755 | - |
purified enzyme, pH and temperature not specified in the publication | Nocardiopsis sp. B2 |
10000 | - |
purified enzyme, pH and temperature not specified in the publication | Anoxybacillus beppuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
corn starch + H2O | - |
Vibrio sp. | ? | - |
? | |
corn starch + H2O | - |
Nocardiopsis sp. B2 | ? | - |
? | |
additional information | a saccharifying enzyme | Bacillus sp. ALSHL3 | ? | - |
? | |
soluble starch + H2O | - |
Bacillus cereus | ? | - |
? | |
starch + H2O | - |
Bacillus subtilis | ? | - |
? | |
starch + H2O | - |
Thermus sp. | ? | - |
? | |
starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
starch + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
starch + H2O | - |
Thermomonospora curvata | ? | - |
? | |
starch + H2O | - |
Eisenia fetida | ? | - |
? | |
starch + H2O | - |
Parageobacillus caldoxylosilyticus | ? | - |
? | |
starch + H2O | - |
Streptomyces coelicolor A3 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. KSM-K38 | ? | - |
? | |
starch + H2O | - |
Bacillus licheniformis | ? | - |
? | |
starch + H2O | - |
Bacillus sp. YX-1 | ? | - |
? | |
starch + H2O | - |
Sutcliffiella cohnii | ? | - |
? | |
starch + H2O | - |
Nesterenkonia sp. | ? | - |
? | |
starch + H2O | - |
Chryseobacterium taeanense | ? | - |
? | |
starch + H2O | - |
Marinobacter sp. EMB8 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. Ferdowsicous | ? | - |
? | |
starch + H2O | - |
Saccharopolyspora sp. A9 | ? | - |
? | |
starch + H2O | - |
Wangia sp. C52 | ? | - |
? | |
starch + H2O | - |
Pseudomonas sp. K6-28-040 | ? | - |
? | |
starch + H2O | - |
Anoxybacillus beppuensis | ? | - |
? | |
starch + H2O | - |
Zunongwangia profunda | ? | - |
? | |
starch + H2O | - |
Rossellomorea aquimaris | ? | - |
? | |
starch + H2O | - |
Bacillus sp. ALSHL3 | ? | - |
? | |
starch + H2O | - |
Streptomyces sp. D1 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. GM8901 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. TS-23 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. I-3 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. TSCVKK | ? | - |
? | |
starch + H2O | - |
Bacillus sp. WN 11 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. A-3-15 | ? | - |
? | |
starch + H2O | - |
Chromohalobacter sp. TVSP 101 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. marini | ? | - |
? | |
starch + H2O | - |
Halobacillus sp. amylus | ? | - |
? | |
starch + H2O | - |
Aureobasidium pullulans N13d | ? | - |
? | |
starch + H2O | - |
Bacillus subtilis AX20 | ? | - |
? | |
starch + H2O | - |
Chryseobacterium taeanense TKU001 | ? | - |
? | |
starch + H2O | - |
Bacillus subtilis DM-03 | ? | - |
? | |
starch + H2O | - |
Anoxybacillus beppuensis TSSC-1 | ? | - |
? | |
starch + H2O | - |
Sutcliffiella cohnii US147 | ? | - |
? | |
starch + H2O | - |
Rossellomorea aquimaris VITP4 | ? | - |
? | |
starch + H2O | - |
Halobacillus sp. amylus HM454199 | ? | - |
? | |
starch + H2O | - |
Parageobacillus caldoxylosilyticus TK4 | ? | - |
? | |
starch + H2O | - |
Nesterenkonia sp. F | ? | - |
? | |
starch + H2O | - |
Streptomyces coelicolor A3 A3(2) | ? | - |
? | |
starch + H2O | - |
Bacillus licheniformis NH1 | ? | - |
? | |
starch + H2O | - |
Streptomyces coelicolor A3 M145 | ? | - |
? | |
starch + H2O | - |
Streptomyces coelicolor A3 ATCC BAA-471 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, about, SDS-PAGE | Wangia sp. C52 |
? | x * 60000, about, SDS-PAGE | Pseudomonas sp. K6-28-040 |
? | x * 70000, about, SDS-PAGE | Saccharopolyspora sp. A9 |
? | x * 70000, about, SDS-PAGE | Streptomyces sp. D1 |
? | x * 62000, about, SDS-PAGE | Thermomonospora curvata |
? | x * 80000, about, SDS-PAGE | Bacillus subtilis |
? | x * 55000, about, SDS-PAGE | Bacillus cereus |
? | x * 55000, about, SDS-PAGE | Vibrio sp. |
? | x * 55000, about, SDS-PAGE | Eisenia fetida |
? | x * 55000, about, SDS-PAGE | Bacillus licheniformis |
? | x * 55000, about, SDS-PAGE | Bacillus sp. I-3 |
? | x * 43000, about, SDS-PAGE | Bacillus subtilis |
? | x * 45000, about, SDS-PAGE | Nocardiopsis sp. B2 |
? | x * 58000, about, SDS-PAGE | Thermus sp. |
? | x * 58000, about, SDS-PAGE | Bacillus sp. KSM-K38 |
? | x * 58000, about, SDS-PAGE | Bacillus sp. YX-1 |
? | x * 65000, about, SDS-PAGE | Anoxybacillus flavithermus |
? | x * 68000, about, SDS-PAGE | Bacillus sp. TS-23 |
? | x * 75000, about, SDS-PAGE | Bacillus sp. ALSHL3 |
Synonyms | Comment | Organism |
---|---|---|
AmyE | - |
Bacillus cereus |
AmyK38 | - |
Bacillus sp. KSM-K38 |
AmyZ2 | - |
Zunongwangia profunda |
B4168_3135 | - |
Anoxybacillus flavithermus |
cold-adapted alpha-amylase | - |
Wangia sp. C52 |
haloalkaline alpha-amylase | - |
Zunongwangia profunda |
maltogenic amylase | UniProt | Parageobacillus caldoxylosilyticus |
salt-tolerant alpha-amylase | - |
Zunongwangia profunda |
salt-tolerant alpha-amylase | - |
Bacillus sp. ALSHL3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Wangia sp. C52 |
40 | - |
- |
Rossellomorea aquimaris |
40 | - |
- |
Bacillus sp. marini |
40 | - |
- |
Halobacillus sp. amylus |
40 | 50 | - |
Bacillus sp. YX-1 |
45 | - |
- |
Nesterenkonia sp. |
45 | - |
- |
Nocardiopsis sp. B2 |
45 | - |
- |
Streptomyces sp. D1 |
50 | - |
- |
Thermus sp. |
50 | - |
- |
Eisenia fetida |
50 | - |
- |
Pseudomonas sp. K6-28-040 |
50 | - |
- |
Zunongwangia profunda |
55 | - |
- |
Bacillus subtilis |
55 | - |
- |
Anoxybacillus flavithermus |
55 | - |
- |
Saccharopolyspora sp. A9 |
55 | 60 | - |
Bacillus sp. KSM-K38 |
60 | - |
- |
Vibrio sp. |
60 | - |
- |
Bacillus sp. ALSHL3 |
60 | - |
- |
Bacillus sp. GM8901 |
60 | - |
- |
Bacillus sp. TS-23 |
65 | - |
- |
Bacillus cereus |
65 | - |
- |
Thermomonospora curvata |
70 | - |
- |
Sutcliffiella cohnii |
70 | - |
- |
Bacillus sp. Ferdowsicous |
70 | - |
- |
Bacillus sp. I-3 |
70 | - |
- |
Bacillus sp. A-3-15 |
75 | 80 | - |
Bacillus sp. WN 11 |
80 | - |
- |
Anoxybacillus beppuensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
reversible unfolding even at high temperature | Rossellomorea aquimaris |
additional information | - |
the purfied enzyme is moderately thermostable | Streptomyces coelicolor A3 |
37 | 100 | purified enzyme, stable at | Anoxybacillus beppuensis |
40 | 45 | purified enzyme, stable at | Anoxybacillus flavithermus |
40 | 60 | purified enzyme, stable at | Zunongwangia profunda |
50 | - |
purified enzyme, stable up to | Vibrio sp. |
60 | - |
stable up to | Bacillus subtilis |
60 | - |
stable up to | Thermus sp. |
65 | - |
purified enzyme, stable up to | Bacillus cereus |
70 | - |
stable up to | Bacillus sp. ALSHL3 |
75 | 85 | purified enzyme, stable at | Nocardiopsis sp. B2 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1449.43 | - |
starch | pH and temperature not specified in the publication | Zunongwangia profunda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Bacillus sp. Ferdowsicous |
5 | - |
- |
Bacillus sp. YX-1 |
5.5 | - |
- |
Eisenia fetida |
5.5 | - |
- |
Bacillus sp. WN 11 |
5.5 | 6.5 | - |
Thermus sp. |
6 | - |
- |
Bacillus subtilis |
6 | - |
- |
Wangia sp. C52 |
6 | - |
- |
Bacillus sp. ALSHL3 |
6.5 | - |
- |
Vibrio sp. |
6.5 | - |
- |
Bacillus licheniformis |
7 | - |
- |
Anoxybacillus flavithermus |
7 | - |
- |
Parageobacillus caldoxylosilyticus |
7 | - |
- |
Marinobacter sp. EMB8 |
7 | - |
- |
Pseudomonas sp. K6-28-040 |
7 | - |
- |
Anoxybacillus beppuensis |
7 | - |
- |
Zunongwangia profunda |
7 | - |
- |
Bacillus sp. I-3 |
7 | - |
- |
Bacillus sp. marini |
7.5 | - |
- |
Nesterenkonia sp. |
7.5 | 9.5 | - |
Rossellomorea aquimaris |
8 | - |
- |
Halobacillus sp. amylus |
8 | 9 | - |
Bacillus sp. KSM-K38 |
8 | 11 | - |
Bacillus cereus |
9 | - |
- |
Bacillus subtilis |
9 | - |
- |
Sutcliffiella cohnii |
9 | - |
- |
Chryseobacterium taeanense |
9 | - |
- |
Nocardiopsis sp. B2 |
9 | - |
- |
Streptomyces sp. D1 |
9 | - |
- |
Bacillus sp. TS-23 |
9 | - |
- |
Chromohalobacter sp. TVSP 101 |
11 | - |
- |
Saccharopolyspora sp. A9 |
11 | - |
- |
Bacillus sp. TSCVKK |
11 | - |
- |
Bacillus sp. A-3-15 |
11 | 12 | - |
Bacillus sp. GM8901 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | stable at | Parageobacillus caldoxylosilyticus |
4 | 9 | stable at | Bacillus licheniformis |
4.5 | 11 | purified enzyme, stable at | Bacillus sp. YX-1 |
5 | 9 | stable at | Bacillus subtilis |
5 | 10 | purified enzyme, stable at | Pseudomonas sp. K6-28-040 |
5 | 10 | purified enzyme, stable at | Anoxybacillus beppuensis |
5.5 | 9 | purified enzyme, stable at | Bacillus sp. WN 11 |
6 | 10 | purified enzyme, stable at | Zunongwangia profunda |
6 | - |
purified enzyme, stable at | Thermomonospora curvata |
6 | 11 | stable at | Bacillus sp. KSM-K38 |
6 | 11 | stable at | Marinobacter sp. EMB8 |
6 | 13 | stable at | Bacillus sp. GM8901 |
6.5 | 7.5 | purified enzyme, stable at | Vibrio sp. |
6.5 | 7.5 | purified enzyme, stable at | Rossellomorea aquimaris |
7 | - |
purified enzyme, stable at | Anoxybacillus flavithermus |
7 | 11 | stable at | Streptomyces sp. D1 |
7 | 11 | purified enzyme, stable at | Nocardiopsis sp. B2 |
7 | 10 | stable at | Chromohalobacter sp. TVSP 101 |
7 | 10 | purified enzyme, stable at | Bacillus sp. TS-23 |
8 | 12 | purified enzyme, stable at | Saccharopolyspora sp. A9 |
9 | - |
stable up to | Sutcliffiella cohnii |
9 | - |
purified enzyme, stable at | Streptomyces coelicolor A3 |
9 | 11 | stable at | Chryseobacterium taeanense |
10 | 12 | stable at | Bacillus sp. A-3-15 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Wangia sp. C52 | - |
- |
5.6 |
Vibrio sp. | - |
- |
6.2 |
Pseudomonas sp. K6-28-040 | - |
7.8 | 7.5 |