Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chloroform | activates by about 40% at 25% v/v | Tepidimonas fonticaldi | |
Cyclohexane | activates by about 15% at 25% v/v | Tepidimonas fonticaldi | |
Dimethyl formamide | activates by about 55% at 25% v/v | Tepidimonas fonticaldi | |
DMSO | activates by 10% at 25% v/v | Tepidimonas fonticaldi | |
ethanol | activates by 15% at 25% v/v | Tepidimonas fonticaldi | |
n-hexane | activates by about 13% at 25% v/v | Tepidimonas fonticaldi |
Cloned (Comment) | Organism |
---|---|
gene amy48, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the extracellular fraction of enzyme TfAmy48 in Escherichia coli strain BL21(DE3)pLysS. The biochemical properties of the extracellular purified recombinant enzyme (rTfAmy48) are similar to those of the native one | Tepidimonas fonticaldi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 59% inhibition at 10 mM | Tepidimonas fonticaldi | |
4-bromophenacyl bromide | 19% inhibition at 2 mM | Tepidimonas fonticaldi | |
Ag+ | inhibits 24% at 5 mM | Tepidimonas fonticaldi | |
Cd2+ | complete inhibition at 5 mM | Tepidimonas fonticaldi | |
Co2+ | inhibits 60% at 5 mM | Tepidimonas fonticaldi | |
Cu2+ | inhibits 75% at 5 mM | Tepidimonas fonticaldi | |
DTT | 74% inhibition at 1 mM | Tepidimonas fonticaldi | |
Hg2+ | complete inhibition at 5 mM | Tepidimonas fonticaldi | |
Mn2+ | inhibits 11% at 5 mM | Tepidimonas fonticaldi | |
additional information | enzyme TfAmy48 shows excellent compatibility with some commercial laundry detergents, overview. No or poor effects by 1 mM PMSF, 2 mM diiodopropyl fluorophosphate, 10 mM EDTA, or 1 mm EGTA | Tepidimonas fonticaldi | |
SDS | 39% inhibition at 60 mM | Tepidimonas fonticaldi | |
Urea | 51% inhibition at 6 M | Tepidimonas fonticaldi | |
Zn2+ | inhibits 79% at 5 mM | Tepidimonas fonticaldi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | classical Michaelis-Menten kinetics, Km for soluble potato starch is 1.22 mg/ml | Tepidimonas fonticaldi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme harbors a predicted signal peptide | Tepidimonas fonticaldi | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates 38% at 5 mM | Tepidimonas fonticaldi | |
Ca2+ | activates 55% at 5 mM | Tepidimonas fonticaldi | |
Fe2+ | activates 22% at 5 mM | Tepidimonas fonticaldi | |
Mg2+ | activates 13% at 5 mM | Tepidimonas fonticaldi | |
Ni2+ | activates 19% at 5 mM | Tepidimonas fonticaldi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
native PAGE and gel filtration | Tepidimonas fonticaldi |
48138 | - |
mass spectrometry | Tepidimonas fonticaldi |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 75% inhibition at 25% v/v | Tepidimonas fonticaldi |
benzene | 55% inhibition at 25% v/v | Tepidimonas fonticaldi |
Ethyl acetate | 55% inhibition at 25% v/v | Tepidimonas fonticaldi |
isooctane | 15% inhibition at 25% v/v | Tepidimonas fonticaldi |
additional information | enzyme TfAmy48 shows extreme stability and tolerance towards organic solvents. After 24 h, TfAmy48 shows a stimulatory effect in the majority of hydrophilic organic solvents, including DMSO, ethanol, chloroform, and DMF with a residual activity of 110, 115, 140, and 155%, respectively. No of poor effects on enzyme activity by 25% v/v of isopropyl alcohol, isopropanol, n-decane, toluene, n-hexanol, n-butanol, and methanol | Tepidimonas fonticaldi |
n-heptane | 25% inhibition at 25% v/v | Tepidimonas fonticaldi |
n-octane | 10% inhibition at 25% v/v | Tepidimonas fonticaldi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tepidimonas fonticaldi | A0A482EWM9 | isolated from a collection obtained froma thermal spring in Hammam Righa commune (GPS coordinates: 36°22' N, 2°23' E), located in Ain Defla province (Algeria). The isolation site is located at an altitude of 525 m, in a mountainous and wooded area. It includes several sources whose water temperature varies from 26°C to 68°C | - |
Tepidimonas fonticaldi HB23 | A0A482EWM9 | isolated from a collection obtained froma thermal spring in Hammam Righa commune (GPS coordinates: 36°22' N, 2°23' E), located in Ain Defla province (Algeria). The isolation site is located at an altitude of 525 m, in a mountainous and wooded area. It includes several sources whose water temperature varies from 26°C to 68°C | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 9.5fold by ammonium sulfate fractionation, heat treatment, gel filtration, and anion exchange chromatography | Tepidimonas fonticaldi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | culture growth in optimized starch broth | Tepidimonas fonticaldi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1275 | - |
purified native extracellular enzyme, substrate soluble starch, pH 8.0, 80°C | Tepidimonas fonticaldi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 75% activity compared to soluble potato starch | Tepidimonas fonticaldi | ? | - |
? | |
amylopectin + H2O | 75% activity compared to soluble potato starch | Tepidimonas fonticaldi HB23 | ? | - |
? | |
amylose + H2O | 85% activity compared to soluble potato starch | Tepidimonas fonticaldi | ? | - |
? | |
amylose + H2O | 85% activity compared to soluble potato starch | Tepidimonas fonticaldi HB23 | ? | - |
? | |
additional information | enzyme TfAmy48 shows an endo-acting pattern. It has no activity with raw starch, alpha-cyclodextrin, and carboxymethyl cellulose | Tepidimonas fonticaldi | ? | - |
? | |
additional information | enzyme TfAmy48 shows an endo-acting pattern. It has no activity with raw starch, alpha-cyclodextrin, and carboxymethyl cellulose | Tepidimonas fonticaldi HB23 | ? | - |
? | |
soluble maize starch + H2O | 60% activity compared to soluble potato starch | Tepidimonas fonticaldi | ? | - |
? | |
soluble maize starch + H2O | 60% activity compared to soluble potato starch | Tepidimonas fonticaldi HB23 | ? | - |
? | |
soluble potato starch + H2O | best substrate | Tepidimonas fonticaldi | maltopentaose + maltose + maltotriose | - |
? | |
soluble potato starch + H2O | best substrate | Tepidimonas fonticaldi HB23 | maltopentaose + maltose + maltotriose | - |
? | |
soluble rice starch + H2O | 55% activity compared to soluble potato starch | Tepidimonas fonticaldi | ? | - |
? | |
soluble wheat starch + H2O | 79% activity compared to soluble potato starch | Tepidimonas fonticaldi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48138, mass spectrometry, 1 * 48000, SDS-PAGE, 1 * 50414, sequence calculation | Tepidimonas fonticaldi |
More | enzyme three-dimensional structure homology modelling, overview | Tepidimonas fonticaldi |
Synonyms | Comment | Organism |
---|---|---|
TfAmy48 | - |
Tepidimonas fonticaldi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | without Ca2+ | Tepidimonas fonticaldi |
80 | - |
in presence of 5 mM Ca2+ | Tepidimonas fonticaldi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified enzyme, 5 mM Ca2+, completely stable for 14 h, loss of 50% activity after 19 h, inactivation after 23 h | Tepidimonas fonticaldi |
70 | - |
purified enzyme, without Ca2+, completely stable for 10 h, loss of 50% activity after 16 h, inactivation after 19 h | Tepidimonas fonticaldi |
80 | - |
purified enzyme, 5 mM Ca2+, completely stable for 8 h, loss of 50% activity after 13 h, inactivation after 16 h | Tepidimonas fonticaldi |
80 | - |
purified enzyme, without Ca2+, completely stable for 6 h, loss of 50% activity after 10 h, inactivation after 13 h | Tepidimonas fonticaldi |
90 | - |
purified enzyme, 5 mM Ca2+, completely stable for 5 h, loss of 50% activity after 8 h, inactivation after 10 h | Tepidimonas fonticaldi |
90 | - |
purified enzyme, without Ca2+, completely stable for 3 h, loss of 50% activity after 6 h, inactivation after 8 h | Tepidimonas fonticaldi |
100 | - |
purified enzyme, 5 mM Ca2+, completely stable for 1 h, loss of 50% activity after 3 h, inactivation after 4 h | Tepidimonas fonticaldi |
100 | - |
purified enzyme, without Ca2+, loss of 50% activity after 2 h, inactivation after 3h | Tepidimonas fonticaldi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
soluble potato starch | pH 8.0, 80°C | Tepidimonas fonticaldi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Tepidimonas fonticaldi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | over 50% of maximal activity within this range, profile overview | Tepidimonas fonticaldi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
purified enzyme, completely stable for 16 h, loss of 50% activity after 21 h, inactivation after 24 h | Tepidimonas fonticaldi |
7 | - |
purified enzyme, completely stable for 12.5 h, loss of 50% activity after 18 h, inactivation after 21 h | Tepidimonas fonticaldi |
8 | - |
purified enzyme, completely stable for 9 h, loss of 50% activity after 15 h, inactivation after 18 h | Tepidimonas fonticaldi |
9 | - |
purified enzyme, completely stable for 8 h, loss of 50% activity after 11 h, inactivation after 13 h | Tepidimonas fonticaldi |
10 | - |
purified enzyme, completely stable for 6 h, loss of 50% activity after 9 h, inactivation after 11 h | Tepidimonas fonticaldi |
11 | - |
purified enzyme, completely stable for 3 h, loss of 50% activity after 6 h, inactivation after 8 h | Tepidimonas fonticaldi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tepidimonas fonticaldi | sequence calculation | - |
6.04 |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme three-dimensional structure homology modelling, overview | Tepidimonas fonticaldi |