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Literature summary for 3.2.1.1 extracted from

  • Du, R.; Song, Q.; Zhang, Q.; Zhao, F.; Kim, R.C.; Zhou, Z.; Han, Y.
    Purification and characterization of novel thermostable and Ca-independent alpha-amylase produced by Bacillus amyloliquefaciens BH072 (2018), Int. J. Biol. Macromol., 115, 1151-1156 .
    View publication on PubMed
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 9-12% activation at 1-5% Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 25% inhibition at 5 mM Bacillus amyloliquefaciens
EDTA
-
 Bacillus amyloliquefaciens
Fe2+ 35% inhibition at 5 mM Bacillus amyloliquefaciens
Hg2+ complete inhibition at 5 mM Bacillus amyloliquefaciens
additional information no or poor inhibition by Ba2+, K+, Ca2+, Mn2+, and Co2+ at 5-50 mM and by SDS, PMSF, Triton X-100, and Tween 20 at 1-5% Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of BH072 alpha-amylase is 4.27 mg/ml Bacillus amyloliquefaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Bacillus amyloliquefaciens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 38% activation at 5 mM Bacillus amyloliquefaciens
additional information BH072 alpha-amylase is a Ca2+-independent enzyme Bacillus amyloliquefaciens
Zn2+ 22% activation at 5 mM Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
 isolated from honey
-
Bacillus amyloliquefaciens BH072
-
 isolated from honey
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain BH072 21.23fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2162.42
-
 purified native enzyme, pH 7.0, 60°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raw starch + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
raw starch + H2O
-
 Bacillus amyloliquefaciens BH072 ?
-
 ?
soluble starch + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
soluble starch + H2O
-
 Bacillus amyloliquefaciens BH072 ?
-
 ?

Synonyms

Synonyms Comment Organism
BH072alpha-amylase
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 55% of maximal activity at 30°C, 80% at 80°C, and 30% at 90°C, profile overview Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, 70% activity remaining after 42 h Bacillus amyloliquefaciens
60
-
 purified enzyme, 95% activity remaining after 42 h Bacillus amyloliquefaciens
70
-
 purified enzyme, 80% activity remaining after 42 h Bacillus amyloliquefaciens
80
-
 purified enzyme, 75% activity remaining after 42 h Bacillus amyloliquefaciens
85
-
 purified enzyme, 25% activity remaining after 42 h Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus amyloliquefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 purified enzyme, 20% activity remaining after 200 min Bacillus amyloliquefaciens
6
-
 purified enzyme, 80% activity remaining after 200 min Bacillus amyloliquefaciens
6.5
-
 purified enzyme, 90% activity remaining after 200 min Bacillus amyloliquefaciens
7
-
 purified enzyme, 95% activity remaining after 200 min Bacillus amyloliquefaciens
7.5
-
 purified enzyme, 90% activity remaining after 200 min Bacillus amyloliquefaciens
8
-
 purified enzyme, 80% activity remaining after 200 min Bacillus amyloliquefaciens
8.5
-
 purified enzyme, 50% activity remaining after 200 min Bacillus amyloliquefaciens
9
-
 purified enzyme, 40% activity remaining after 200 min Bacillus amyloliquefaciens

General Information

General Information Comment Organism
additional information N-terminal amino acid sequencing using mass spectrometry for analysis and homology modelling of the catalytic domain, overview Bacillus amyloliquefaciens