Cloned (Comment) | Organism |
---|---|
expression in REscherichia coli | Acidithiobacillus ferrooxidans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | outer membrane | Acidithiobacillus ferrooxidans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | Q0KK37 | - |
- |
Purification (Comment) | Organism |
---|---|
expression in Escherichia coli results in the formation of inclusion bodies of the protein in an inactive form. The recombinant protein can be successfully activated after an in vitro refolding treatment. The enzyme requires exposure to an acidicenvironment during protein folding for activation | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
recombinant protein, pH 3.0, 30°C | Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required during the refolding process, no cofoactor required for activity | Acidithiobacillus ferrooxidans |