BRENDA - Enzyme Database show
show all sequences of 3.12.1.1

Purification and partial characterization from the acidophilic sulphur oxidizer Thiobacillus acidophilus

Meulenberg, R.; Pronk, J.T.; Frank, J.; Hazeu, W.; Bos, P.; Kuenen, J.G.; Eur. J. Biochem. 209, 367-374 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
sulfate
maximum activity at 1.4-2.0 M
Acidiphilium acidophilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
-
Acidiphilium acidophilum
N-ethylmaleimide
-
Acidiphilium acidophilum
tetrathionate
-
Acidiphilium acidophilum
thiosulfate
-
Acidiphilium acidophilum
trithionate
substrate inhibition, Ki: 6 mM
Acidiphilium acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
trithionate
at 30°C
Acidiphilium acidophilum
0.27
-
trithionate
at 70°C
Acidiphilium acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Acidiphilium acidophilum
99000
-
gel filtration
Acidiphilium acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trithionate + H2O
Acidiphilium acidophilum
involved in cytochrome c reduction
sulfate + thiosulfate
-
Acidiphilium acidophilum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidiphilium acidophilum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acidiphilium acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trithionate + H2O
-
288770
Acidiphilium acidophilum
sulfate + thiosulfate
-
288770
Acidiphilium acidophilum
?
trithionate + H2O
involved in cytochrome c reduction
288770
Acidiphilium acidophilum
sulfate + thiosulfate
-
288770
Acidiphilium acidophilum
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 34000
Acidiphilium acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Acidiphilium acidophilum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
90
-
20% of maximum activity
Acidiphilium acidophilum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
no loss of activity by frequent freezing and thawing
Acidiphilium acidophilum
-
-
activity lost after 36 days
Acidiphilium acidophilum
30
-
40% activity after 97 days
Acidiphilium acidophilum
70
-
activity lost after 27 days
Acidiphilium acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4.5
-
Acidiphilium acidophilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
1
7.5
active above pH 1 and below pH 7.5
Acidiphilium acidophilum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
sulfate
maximum activity at 1.4-2.0 M
Acidiphilium acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
-
Acidiphilium acidophilum
N-ethylmaleimide
-
Acidiphilium acidophilum
tetrathionate
-
Acidiphilium acidophilum
thiosulfate
-
Acidiphilium acidophilum
trithionate
substrate inhibition, Ki: 6 mM
Acidiphilium acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
trithionate
at 30°C
Acidiphilium acidophilum
0.27
-
trithionate
at 70°C
Acidiphilium acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Acidiphilium acidophilum
99000
-
gel filtration
Acidiphilium acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trithionate + H2O
Acidiphilium acidophilum
involved in cytochrome c reduction
sulfate + thiosulfate
-
Acidiphilium acidophilum
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acidiphilium acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trithionate + H2O
-
288770
Acidiphilium acidophilum
sulfate + thiosulfate
-
288770
Acidiphilium acidophilum
?
trithionate + H2O
involved in cytochrome c reduction
288770
Acidiphilium acidophilum
sulfate + thiosulfate
-
288770
Acidiphilium acidophilum
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 34000
Acidiphilium acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Acidiphilium acidophilum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
90
-
20% of maximum activity
Acidiphilium acidophilum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
no loss of activity by frequent freezing and thawing
Acidiphilium acidophilum
-
-
activity lost after 36 days
Acidiphilium acidophilum
30
-
40% activity after 97 days
Acidiphilium acidophilum
70
-
activity lost after 27 days
Acidiphilium acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4.5
-
Acidiphilium acidophilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
1
7.5
active above pH 1 and below pH 7.5
Acidiphilium acidophilum
Other publictions for EC 3.12.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701172
Anandham
Chemolithoautotrophic oxidatio ...
Alcaligenes sp., Alcaligenes sp. ATSB22, Dyella ginsengisoli, Dyella ginsengisoli ATSB10, Halothiobacillus sp., Halothiobacillus sp. ATSB2, Leifsonia shinshuensis, Leifsonia shinshuensis ATSB20, Methylobacterium phyllosphaerae, Methylobacterium phyllosphaerae ATSB31, Pandoraea pnomenusa, Pandoraea pnomenusa ATSB26, Pandoraea sp., Pandoraea sp. ATSB30, Pandoraea sputorum, Pandoraea sputorum ATSB28, Paraburkholderia kururiensis
Res. Microbiol.
159
579-589
2008
-
-
-
-
-
-
-
-
-
-
-
17
-
31
-
-
-
-
-
-
9
-
34
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
-
-
-
9
-
34
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288770
Meulenberg
Purification and partial chara ...
Acidiphilium acidophilum
Eur. J. Biochem.
209
367-374
1992
1
-
-
-
-
-
5
2
-
-
2
1
-
2
-
-
1
-
-
-
-
-
2
1
1
1
4
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
2
-
-
2
1
-
-
-
1
-
-
-
-
2
1
1
1
4
-
1
1
-
-
-
-
-
-
-
-
288769
Lu
-
Cellular location and partial ...
Thermithiobacillus tepidarius
J. Gen. Microbiol.
134
877-885
1988
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-