BRENDA - Enzyme Database
show all sequences of 3.1.99.B2

Crystallization of the xeroderma pigmentosum group F endonuclease from Aeropyrum pernix

Lally, J.; Newman, M.; Murray-Rust, J.; Fadden, A.; Kawarabayasi, Y.; McDonald, N.; Acta Crystallogr. Sect. D 60, 1658-1661 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Aeropyrum pernix
Crystallization (Commentary)
Crystallization
Organism
three different crystal forms of the enzyme are grown in trigonal, monoclinic and triclinic systems. The trigonal crystals diffract to 2.8 A and are grown in the presence of double-stranded DNA. Monoclinic crystals are grown without DNA and diffract to 3.2 A
Aeropyrum pernix
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aeropyrum pernix
Q9YC15
-
-
Purification (Commentary)
Commentary
Organism
-
Aeropyrum pernix
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Aeropyrum pernix
Crystallization (Commentary) (protein specific)
Crystallization
Organism
three different crystal forms of the enzyme are grown in trigonal, monoclinic and triclinic systems. The trigonal crystals diffract to 2.8 A and are grown in the presence of double-stranded DNA. Monoclinic crystals are grown without DNA and diffract to 3.2 A
Aeropyrum pernix
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aeropyrum pernix
Other publictions for EC 3.1.99.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719360
Newman
Structure of an XPF endonuclea ...
Aeropyrum pernix
EMBO J.
24
895-905
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
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-
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1
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-
-
-
-
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-
-
-
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-
1
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
719834
Roberts
An archaeal endonuclease displ ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
280
5924-5928
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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2
-
1
-
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1
-
-
-
-
-
-
-
-
-
720574
Roberts
DNA end-directed and processiv ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Nucleic Acids Res.
33
6662-6670
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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2
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2
-
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-
718472
Lally
Crystallization of the xeroder ...
Aeropyrum pernix
Acta Crystallogr. Sect. D
60
1658-1661
2004
-
-
1
1
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-
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-
3
-
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1
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1
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1
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1
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720486
Roberts
An archaeal XPF repair endonuc ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Mol. Microbiol.
48
361-371
2003
1
-
1
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-
-
-
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8
-
-
1
-
-
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2
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1
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1
1
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1
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1
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1
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2
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1
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1
1
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