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Literature summary for 3.1.8.2 extracted from
- Hydrolysis of organophosphorus compounds by microbial enzymes (2011), Appl. Microbiol. Biotechnol., 89, 35-43.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | biosensors using immobilized recombinant Escherichia coli cells expressing OPH are being employed for identifying OP nerve agents | Flavobacterium sp. |
| analysis | biosensors using immobilized recombinant Escherichia coli cells expressing OPH are being employed for identifying OP nerve agents | Brevundimonas diminuta |
| environmental protection | detoxification of nerve agent exposed environments | Alteromonas sp. |
| environmental protection | detoxification of nerve agent exposed environments | Pseudoalteromonas haloplanktis |
| environmental protection | detoxification of nerve agent exposed environments | Pseudoalteromonas undina |
| environmental protection | to detoxify nerve agent exposed environments, a decontamination solution known as DS2 is being used in conjunction with bleach | Flavobacterium sp. |
| environmental protection | to detoxify nerve agent exposed environments, a decontamination solution known as DS2 is being used in conjunction with bleach | Brevundimonas diminuta |
| medicine | OPH is also being used in medical applications as an antidote or a therapeutic in preventing organophosphorous poisoning | Flavobacterium sp. |
| medicine | OPH is also being used in medical applications as an antidote or a therapeutic in preventing organophosphorous poisoning | Brevundimonas diminuta |
| additional information | the OPH enzyme is incorporated successfully into fire-fighting foams for large-scale response cleanup studies of contaminated areas | Flavobacterium sp. |
| additional information | the OPH enzyme is incorporated successfully into fire-fighting foams for large-scale response cleanup studies of contaminated areas | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H254R | the active site mutation results in increased activity with soman and VX | Brevundimonas diminuta |
| H275L | the active site mutation results in increased activity with soman and VX | Brevundimonas diminuta |
| H275V | the active site mutation results in increased activity with soman and VX | Brevundimonas diminuta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta |  |
| 0.058 | - |
paraoxon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.08 | - |
methyl parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.24 | - |
parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.39 | - |
Coumaphos | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.43 | - |
O-ethyl S-(2-diisopropylamino)ethyl methylphosphonothioate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.45 | - |
diazinon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.46 | - |
fensulfothion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.5 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 0.68 | - |
O-cyclohexyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 0.7 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 1.27 | - |
paraoxon | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 1.57 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 2.48 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 2.99 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | activates | Flavobacterium sp. | |
| Cd2+ | activates | Brevundimonas diminuta | |
| Co2+ | activates | Flavobacterium sp. | |
| Co2+ | activates | Brevundimonas diminuta | |
| Mn2+ | activates | Flavobacterium sp. | |
| Mn2+ | activates | Brevundimonas diminuta | |
| Mn2+ | activates | Alteromonas sp. | |
| Mn2+ | activates | Pseudoalteromonas haloplanktis | |
| Mn2+ | activates | Pseudoalteromonas undina | |
| additional information | activity is driven by a binuclear metal center in the C-terminal region | Flavobacterium sp. | |
| additional information | activity is driven by a binuclear metal center in the C-terminal region | Brevundimonas diminuta | |
| additional information | activity is driven by a binuclear metal center in the C-terminal region | Alteromonas sp. | |
| additional information | activity is driven by a binuclear metal center in the C-terminal region | Pseudoalteromonas haloplanktis | |
| additional information | activity is driven by a binuclear metal center in the C-terminal region | Pseudoalteromonas undina | |
| Ni2+ | activates | Flavobacterium sp. | |
| Ni2+ | activates | Brevundimonas diminuta | |
| Zn2+ | required, best activating divalent cation | Flavobacterium sp. | |
| Zn2+ | required, best activating divalent cation | Brevundimonas diminuta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, OPH from displays the TIM barrel fold in the active site loaded with Zn2+, overview | Brevundimonas diminuta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Flavobacterium sp. | the enzyme shows broad substrate specificity and is able to degrade organophosphorus compounds with P-O, P-CN, and P-F bonds and is the only enzyme known to cleave the P-S bond, which is characteristic of V-type nerve agents such as VX | ? | - |
? | |
| additional information | Brevundimonas diminuta | the enzyme shows broad substrate specificity and is able to degrade organophosphorus compounds with P-O, P-CN, and P-F bonds and is the only enzyme known to cleave the P-S bond, which is characteristic of V-type nerve agents such as VX | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alteromonas sp. | - |
- |
- |
| Alteromonas sp. JD6.5 | - |
- |
- |
| Brevundimonas diminuta | - |
gene opd | - |
| Flavobacterium sp. | - |
gene opd | - |
| Pseudoalteromonas haloplanktis | - |
- |
- |
| Pseudoalteromonas undina | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coumaphos + H2O | - |
Brevundimonas diminuta | ? | - |
? | |
| diazinon + H2O | - |
Brevundimonas diminuta | ? | - |
? | |
| diisopropyl fluorophosphate + H2O | - |
Brevundimonas diminuta | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl fluorophosphate + H2O | - |
Alteromonas sp. | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl fluorophosphate + H2O | - |
Alteromonas sp. JD6.5 | diisopropyl phosphate + fluoride | - |
? | |
| ethyl N,N-dimethylphosphoramidocyanidate + H2O | i.e. tabun | Brevundimonas diminuta | ethyl N,N-dimethylphosphoramide + cyanide | - |
? | |
| fensulfothion + H2O | - |
Brevundimonas diminuta | ? | - |
? | |
| methyl parathion + H2O | - |
Brevundimonas diminuta | ? | - |
? | |
| additional information | the enzyme shows broad substrate specificity and is able to degrade organophosphorus compounds with P-O, P-CN, and P-F bonds and is the only enzyme known to cleave the P-S bond, which is characteristic of V-type nerve agents such as VX | Flavobacterium sp. | ? | - |
? | |
| additional information | the enzyme shows broad substrate specificity and is able to degrade organophosphorus compounds with P-O, P-CN, and P-F bonds and is the only enzyme known to cleave the P-S bond, which is characteristic of V-type nerve agents such as VX | Brevundimonas diminuta | ? | - |
? | |
| additional information | OPH is able to degrade a broad list of some of the most toxic organophosphorous pesticides, such as paraoxon, and OP nerve agents including DFP, sarin, and soman | Brevundimonas diminuta | ? | - |
? | |
| additional information | the ability of OPAA to cleave both G-type nerve agents comes from the hydroxide ion in the metal center that facilitates nucleophilic attack on either the carbonyl oxygen of the phosphorus center of organophosphorus nerve agents. The enzyme also shows prolidase activity | Alteromonas sp. | ? | - |
? | |
| additional information | the ability of OPAA to cleave both G-type nerve agents comes from the hydroxide ion in the metal center that facilitates nucleophilic attack on either the carbonyl oxygen of the phosphorus center of organophosphorus nerve agents. The enzyme also shows prolidase activity | Pseudoalteromonas haloplanktis | ? | - |
? | |
| additional information | the ability of OPAA to cleave both G-type nerve agents comes from the hydroxide ion in the metal center that facilitates nucleophilic attack on either the carbonyl oxygen of the phosphorus center of organophosphorus nerve agents. The enzyme also shows prolidase activity | Pseudoalteromonas undina | ? | - |
? | |
| additional information | the ability of OPAA to cleave both G-type nerve agents comes from the hydroxide ion in the metal center that facilitates nucleophilic attack on either the carbonyl oxygen of the phosphorus center of organophosphorus nerve agents. The enzyme also shows prolidase activity | Alteromonas sp. JD6.5 | ? | - |
? | |
| O-cyclohexyl methylphosphonofluoridate + H2O | i.e. cyclosarin | Brevundimonas diminuta | O-cyclohexyl methylphosphate + fluoride | - |
? | |
| O-cyclohexyl methylphosphonofluoridate + H2O | i.e. cyclosarin | Alteromonas sp. | O-cyclohexyl methylphosphate + fluoride | - |
? | |
| O-cyclohexyl methylphosphonofluoridate + H2O | i.e. cyclosarin | Alteromonas sp. JD6.5 | O-cyclohexyl methylphosphate + fluoride | - |
? | |
| O-ethyl S-(2-diisopropylamino)ethyl methylphosphonothioate + H2O | an organophosphorous nerve agent | Brevundimonas diminuta | ? | - |
? | |
| O-isopropylmethylphosphonofluoridate + H2O | i.e. sarin | Brevundimonas diminuta | O-isopropylmethylphosphate + fluoride | - |
? | |
| O-isopropylmethylphosphonofluoridate + H2O | i.e. sarin | Alteromonas sp. | O-isopropylmethylphosphate + fluoride | - |
? | |
| O-isopropylmethylphosphonofluoridate + H2O | i.e. sarin | Alteromonas sp. JD6.5 | O-isopropylmethylphosphate + fluoride | - |
? | |
| O-pinacolyl methylphosphonofluoridate + H2O | i.e. soman | Brevundimonas diminuta | O-pinacolyl methylphosphate + fluoride | - |
? | |
| O-pinacolyl methylphosphonofluoridate + H2O | i.e. soman | Alteromonas sp. | O-pinacolyl methylphosphate + fluoride | - |
? | |
| paraoxon + H2O | - |
Brevundimonas diminuta | diethylphosphate + 4-nitrophenol | - |
? | |
| paraoxon + H2O | - |
Alteromonas sp. | diethylphosphate + 4-nitrophenol | - |
? | |
| paraoxon + H2O | - |
Alteromonas sp. JD6.5 | diethylphosphate + 4-nitrophenol | - |
? | |
| parathion + H2O | - |
Brevundimonas diminuta | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, OPH from displays the TIM barrel fold in the active site loaded with Zn2+, overview | Brevundimonas diminuta |
| tetramer | active enzyme | Pseudoalteromonas haloplanktis |
| tetramer | active enzyme | Pseudoalteromonas undina |
| tetramer | active enzyme, OPAA/prolidase from Alteromonas sp. JD6.5 displays the pita bread fold in the C-terminal region which houses the active metal site loaded with Mn2+, overview | Alteromonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPAA | - |
Alteromonas sp. |
| OPAA | - |
Pseudoalteromonas haloplanktis |
| OPAA | - |
Pseudoalteromonas undina |
| OPH | OPH is a member of the amidohydrolase superfamily | Flavobacterium sp. |
| OPH | OPH is a member of the amidohydrolase superfamily | Brevundimonas diminuta |
| organophosphorus acid anhydrolase | - |
Alteromonas sp. |
| organophosphorus acid anhydrolase | - |
Pseudoalteromonas haloplanktis |
| organophosphorus acid anhydrolase | - |
Pseudoalteromonas undina |
| organophosphorus hydrolase | - |
Flavobacterium sp. |
| organophosphorus hydrolase | - |
Brevundimonas diminuta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
O-ethyl S-(2-diisopropylamino)ethyl methylphosphonothioate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 5 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 6.11 | - |
paraoxon | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 56 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 67 | - |
fensulfothion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 151 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 176 | - |
diazinon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 189 | - |
methyl parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 230 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 442 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 465 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 610 | - |
Coumaphos | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 630 | - |
parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 652 | - |
O-cyclohexyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 3170 | - |
paraoxon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | organophosphorus hydrolase, OPH, contains a two-oxygen bridging mechanism in the active site suggesting subtle differences compared to organophosphorus acid anhydrolase, OPAA. OPAAs exhibit higher soman activities, whereas OPHs have higher activity against the organophosphorus pesticide paraoxon | Flavobacterium sp. |
| additional information | organophosphorus hydrolase, OPH, contains a two-oxygen bridging mechanism in the active site suggesting subtle differences compared to organophosphorus acid anhydrolase, OPAA. OPAAs exhibit higher soman activities, whereas OPHs have higher activity against the organophosphorus pesticide paraoxon | Brevundimonas diminuta |
| additional information | organophosphorus hydrolase, OPH, contains a two-oxygen bridging mechanism in the active site suggesting subtle differences compared to organophosphorus acid anhydrolase, OPAA. OPAAs exhibit higher soman activities, whereas OPHs have higher activity against the organophosphorus pesticide paraoxon | Alteromonas sp. |
| additional information | organophosphorus hydrolase, OPH, contains a two-oxygen bridging mechanism in the active site suggesting subtle differences compared to organophosphorus acid anhydrolase, OPAA. OPAAs exhibit higher soman activities, whereas OPHs have higher activity against the organophosphorus pesticide paraoxon | Pseudoalteromonas haloplanktis |
| additional information | organophosphorus hydrolase, OPH, contains a two-oxygen bridging mechanism in the active site suggesting subtle differences compared to organophosphorus acid anhydrolase, OPAA. OPAAs exhibit higher soman activities, whereas OPHs have higher activity against the organophosphorus pesticide paraoxon | Pseudoalteromonas undina |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
O-ethyl S-(2-diisopropylamino)ethyl methylphosphonothioate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 10 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 61 | - |
O-pinacolyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 77 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 80 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 150 | - |
fensulfothion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 282 | - |
O-isopropylmethylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 390 | - |
diazinon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 959 | - |
O-cyclohexyl methylphosphonofluoridate | pH not specified in the publication, temperature not specified in the publication | Alteromonas sp. | |
| 1600 | - |
Coumaphos | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 2400 | - |
methyl parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 2600 | - |
parathion | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 9700 | - |
diisopropyl fluorophosphate | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
| 55000 | - |
paraoxon | pH not specified in the publication, temperature not specified in the publication | Brevundimonas diminuta | |
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