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Literature summary for 3.1.8.2 extracted from

  • Kondo, Y.; Ishigami, A.; Kubo, S.; Handa, S.; Gomi, K.; Hirokawa, K.; Kajiyama, N.; Chiba, T.; Shimokado, K.; Maruyama, N.
    Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver (2004), FEBS Lett., 570, 57-62.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CdCl2 1 mM, stimulates hydrolysis to a lesser extent Mus musculus
CdCl2 1 mM, stimulates hydrolysis to a lesser extent, 38% activity, compared to MgCl2 Rattus norvegicus
CoCl2 1 mM, stimulates hydrolysis well Mus musculus
CoCl2 1 mM, stimulates hydrolysis well, 102% activity, compared to MgCl2 Rattus norvegicus
MgCl2 1 mM, stimulates hydrolysis well Mus musculus
MgCl2 1 mM, stimulates hydrolysis well, 100% activity Rattus norvegicus
MnCl2 1 mM, stimulates hydrolysis well Mus musculus
MnCl2 1 mM, stimulates hydrolysis well, 100% activity Rattus norvegicus

Application

Application Comment Organism
additional information the enzyme protects the liver from toxic effects of the substrate diisopropyl phosphorofluoridate Mus musculus
additional information the enzyme protects the liver from toxic effects of the substrate diisopropyl phosphorofluoridate Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of SMP30 knockout mice showing no activity with diisopropyl phosphofluoridate in livers, the liver of the mutant mice is far more susceptible to cytotoxicity of diisopropyl phosphofluoridate Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ slightly activates the hydrolysis of diisopropyl phosphofluoridate Rattus norvegicus
Co2+ fully activates the hydrolysis of diisopropyl phosphofluoridate Rattus norvegicus
Mg2+ fully activates the hydrolysis of diisopropyl phosphofluoridate Rattus norvegicus
Mn2+ fully activates the hydrolysis of diisopropyl phosphofluoridate Rattus norvegicus
additional information no effect by Ca2+ on SMP30, the enzyme does not bind to Ca2+ at all Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Rattus norvegicus
34000
-
x * 34000, SMP30, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diisopropyl phosphofluoridate + H2O Mus musculus SMP30 is important in diisopropyl phosphofluoridate detoxification diisopropyl phosphate + fluoride
-
?
diisopropyl phosphofluoridate + H2O Rattus norvegicus SMP30 is important in diisopropyl phosphofluoridate detoxification diisopropyl phosphate + fluoride
-
?
additional information Rattus norvegicus enzyme expression in liver decreases androgen-independently of aging ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus norvegicus
-
-
-
Rattus norvegicus Q03336 SMP30, gene rgn; gene rgn
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus
-
Rattus norvegicus
SMP30 49fold from liver by anion exchange and hydrophobic interaction chromatography, and gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
Mus musculus
-
liver
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver SMP30 Mus musculus
-
liver SMP30 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.527
-
purified SMP30, substrate phenyl acetate Rattus norvegicus
0.527
-
with phenyl acetate as substrate, pH 8.0, 25┬░C, 1 mM MgCl2 Rattus norvegicus
0.998
-
with diisopropyl phosphorofluoridate as substrate, pH 8.0, 25┬░C, 1 mM MgCl2 Rattus norvegicus
1.512
-
purified enzyme Rattus norvegicus
1.512
-
purified SMP30, substrate diisopropyl phosphofluoridate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diisopropyl phosphofluoridate + H2O
-
Rattus norvegicus diisopropyl phosphate + fluoride
-
?
diisopropyl phosphofluoridate + H2O SMP30 is important in diisopropyl phosphofluoridate detoxification Mus musculus diisopropyl phosphate + fluoride
-
?
diisopropyl phosphofluoridate + H2O SMP30 is important in diisopropyl phosphofluoridate detoxification Rattus norvegicus diisopropyl phosphate + fluoride
-
?
diisopropyl phosphofluoridate + H2O substrate of SMP30 Mus musculus diisopropyl phosphate + fluoride
-
?
diisopropyl phosphorofluoridate + H2O pH 8.0, 25┬░C, 1 mM MgCl2 Mus musculus diisopropyl phosphate + fluoride
-
?
diisopropyl phosphorofluoridate + H2O pH 8.0, 25┬░C, 1 mM MgCl2 Rattus norvegicus diisopropyl phosphate + fluoride
-
?
additional information enzyme expression in liver decreases androgen-independently of aging Rattus norvegicus ?
-
?
additional information no activity of SMP30 with paraoxon, dihydrocoumarin, gamma-nonalactone, and delta-dodecanolactone Mus musculus ?
-
?
additional information no activity of SMP30 with paraoxon, dihydrocoumarin, gamma-nonalactone, and delta-dodecanolactone Rattus norvegicus ?
-
?
additional information no activity with paraoxon, dihydrocoumarin, gamma-nonalactone and delta-dodecanolactone Rattus norvegicus ?
-
?
phenyl acetate + H2O
-
Rattus norvegicus phenol + acetate
-
?
phenyl acetate + H2O pH 8.0, 25┬░C, 1 mM MgCl2 Rattus norvegicus phenol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Rattus norvegicus
? x * 34000, SMP30, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
DFPase
-
Mus musculus
DFPase
-
Rattus norvegicus
More SMP30 does not belong to the PON family Mus musculus
More SMP30 does not belong to the PON family Rattus norvegicus
RC
-
Rattus norvegicus
regucalcin
-
Rattus norvegicus
senescence marker protein-30
-
Mus musculus
senescence marker protein-30
-
Rattus norvegicus
SMP30
-
Mus musculus
SMP30
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rattus norvegicus