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Literature summary for 3.1.8.2 extracted from

  • Katsemi, V.; Lucke, C.; Koepke, J.; Lohr, F.; Maurer, S.; Fritzsch, G.; Ruterjans, H.
    Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme (2005), Biochemistry, 44, 9022-9033.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Loligo vulgaris

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutant N175D, 1.7 A resolution Loligo vulgaris

Protein Variants

Protein Variants Comment Organism
D121F no activity Loligo vulgaris
D229N/N120D no activity Loligo vulgaris
D232S 3% higher activity than the wild-type Loligo vulgaris
F173A 84% lower activity than the wild-type Loligo vulgaris
F173L 28% lower activity than the wild-type Loligo vulgaris
F173S 68% lower activity than the wild-type Loligo vulgaris
F173V 46% lower activity than the wild-type Loligo vulgaris
F173W 19% lower activity than the wild-type Loligo vulgaris
F173Y 53% lower activity than the wild-type Loligo vulgaris
F314A 3% higher activity than the wild-type Loligo vulgaris
H181N 19% lower activity than the wild-type Loligo vulgaris
H274N 7% lower activity than the wild-type Loligo vulgaris
H287A 90% lower activity than the wild-type Loligo vulgaris
H287D 99% lower activity than the wild-type Loligo vulgaris
H287F 36% lower activity than the wild-type Loligo vulgaris
H287L 21% lower activity than the wild-type Loligo vulgaris
H287N 96% lower activity than the wild-type Loligo vulgaris
H287Q 54% lower activity than the wild-type Loligo vulgaris
H287W 34% lower activity than the wild-type Loligo vulgaris
H287Y 57% lower activity than the wild-type Loligo vulgaris
M148A 26% lower activity than the wild-type Loligo vulgaris
N120D 96% lower activity than the wild-type Loligo vulgaris
N175D 98% lower activity than the wild-type Loligo vulgaris
N237S 4% lower activity than the wild-type Loligo vulgaris
N272F no activity Loligo vulgaris
Q304F 50% lower activity than the wild-type Loligo vulgaris
Q304W 3% lower activity than the wild-type Loligo vulgaris
Q77F no activity Loligo vulgaris
Q77W 6% higher activity than the wild-type Loligo vulgaris
Q77Y 6% lower activity than the wild-type Loligo vulgaris
R146S 45% lower activity than the wild-type Loligo vulgaris
S271A 34% higher activity than the wild-type Loligo vulgaris
S271A/D232S 19% lower activity than the wild-type Loligo vulgaris
T195A 60% lower activity than the wild-type Loligo vulgaris
T195L 11% lower activity than the wild-type Loligo vulgaris
T195V 3% lower activity than the wild-type Loligo vulgaris
Y144S 8% higher activity than the wild-type Loligo vulgaris

Organism

Organism UniProt Comment Textmining
Loligo vulgaris Q7SIG4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Loligo vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
4
-
mutant N175D Loligo vulgaris
7
-
mutant N120D Loligo vulgaris
19
-
mutant H287A Loligo vulgaris
31
-
mutant F173A Loligo vulgaris
63
-
mutant F173S Loligo vulgaris
78
-
mutant T195A Loligo vulgaris
84
-
mutant H287Y Loligo vulgaris
90
-
mutant H287Q Loligo vulgaris
92
-
mutant F173Y Loligo vulgaris
97
-
mutant Q304F Loligo vulgaris
105
-
mutant F173V Loligo vulgaris
106
-
mutant R146S Loligo vulgaris
124
-
mutant H287F Loligo vulgaris
129
-
mutant H287W Loligo vulgaris
143
-
mutant F173L Loligo vulgaris
143
-
mutant M148A Loligo vulgaris
154
-
mutant H287L Loligo vulgaris
158
-
mutant F173W Loligo vulgaris
158
-
mutant S271A/D232S Loligo vulgaris
173
-
mutant T195L Loligo vulgaris
174
-
mutant Q77Y Loligo vulgaris
187
-
mutant N237S Loligo vulgaris
188
-
mutant Q304W Loligo vulgaris
188
-
mutant T195V Loligo vulgaris
193
-
mutant Q77W Loligo vulgaris
194
-
wild-type Loligo vulgaris
199
-
mutant F314A Loligo vulgaris
200
-
mutant D232S Loligo vulgaris
209
-
mutant Y144S Loligo vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diisopropyl fluorophosphate + H2O room temperature, pH 7.5 Loligo vulgaris diisopropyl phosphate + fluoride
-
?

Synonyms

Synonyms Comment Organism
DFPase
-
Loligo vulgaris
diisopropylfluorophosphatase
-
Loligo vulgaris