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Literature summary for 3.1.8.2 extracted from

  • Cheng, T.C.; Harvey, S.P.; Stroup, A.N.
    Purification and properties of a highly active organophosphorus acid anhydrolase from Alteromonas undina (1993), Appl. Environ. Microbiol., 59, 3138-3140.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation, less effective than DTT Pseudoalteromonas undina
DTT activation Pseudoalteromonas undina

General Stability

General Stability Organism
2-mercaptoethanol enhances stability Pseudoalteromonas undina
DTT enhances stability Pseudoalteromonas undina

Inhibitors

Inhibitors Comment Organism Structure
EGTA Mn2+ reverses and stimulates over pre-EGTA-treatment activity level, Co2+ partially restores; reversible by Mn2+ or Co2+; strong Pseudoalteromonas undina
Fe2+ weak Pseudoalteromonas undina
iodoacetic acid
-
Pseudoalteromonas undina
N-ethylmaleimide
-
Pseudoalteromonas undina
Ni2+ weak Pseudoalteromonas undina
Zn2+
-
Pseudoalteromonas undina

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Pseudoalteromonas undina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
1 * 53000 SDS-PAGE Pseudoalteromonas undina

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas undina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudoalteromonas undina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
327.8
-
-
Pseudoalteromonas undina
575
-
-
Pseudoalteromonas undina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O i.e. 4-nitrophenylethyl phenylphosphinate Pseudoalteromonas undina ?
-
?
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O at 46% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
4-nitrophenyl-methyl(phenyl)phosphinate + H2O i.e. 4-nitrophenylmethyl phenylphosphinate Pseudoalteromonas undina ?
-
?
4-nitrophenyl-methyl(phenyl)phosphinate + H2O at 28% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
4-nitrophenylisopropyl phenylphosphinate + H2O at 22% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
4-nitrophenylpropyl phenylphosphinate + H2O at 24% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
diisopropyl fluorophosphate + H2O at about 30% of O-pinacolylmethylphosphonofluoridate hydrolysis Pseudoalteromonas undina diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Pseudoalteromonas undina diisopropyl phosphate + fluoride
-
?
mipafox + H2O i.e. N,N'-diisopropyl phosphorodiamidofluoridate, poor substrate Pseudoalteromonas undina ?
-
?
additional information not: 4-nitrophenylphosphate Pseudoalteromonas undina ?
-
?
additional information 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate Pseudoalteromonas undina ?
-
?
O-cyclohexylmethylphosphonofluoridate + H2O at 114% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
O-pinacolylmethylphosphonofluoridate + H2O best substrate Pseudoalteromonas undina ?
-
?
paraoxon + H2O i.e. diethyl-4-nitrophenyl phosphate, poor substrate Pseudoalteromonas undina diethylphosphate + 4-nitrophenol
-
?
sarin + H2O at 38% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?
tabun + H2O i.e. N,N-dimethylethylphosphoramidocyanidate, at 124% the rate of diisopropyl fluorophosphate hydrolysis Pseudoalteromonas undina ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000 SDS-PAGE Pseudoalteromonas undina

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Pseudoalteromonas undina
55
-
-
Pseudoalteromonas undina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
917
-
diisopropyl fluorophosphate value above Pseudoalteromonas undina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudoalteromonas undina