Cloned (Comment) | Organism |
---|---|
recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells | Homo sapiens |
recombinant expression of full-length enzyme in Escherichia coli and in S2 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Mesh1 deletion by imprecise excision of a P-element from the Drosophila Mesh1 G3858 allele, resulting in the Drosophila Mesh1 null allele, Mesh1 5A3. The Mesh1 null mutant is much more susceptible than wild-type flies to death from amino acid starvation, and this susceptibility is also completely rescued by Mesh1 expression | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, but less effective than Mn2+ | Drosophila melanogaster | |
Mn2+ | activates, required | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1 | ? | - |
? | |
ppGpp + H2O | Drosophila melanogaster | - |
ppG + diphosphate | - |
? | |
ppGpp + H2O | Homo sapiens | - |
ppG + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant full-length enzyme from Escherichia coli | Drosophila melanogaster |
recombinant full-length enzyme from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1 | Homo sapiens | ? | - |
? | |
additional information | activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview | Drosophila melanogaster | ? | - |
? | |
additional information | activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview | Homo sapiens | ? | - |
? | |
ppGpp + H2O | - |
Drosophila melanogaster | ppG + diphosphate | - |
? | |
ppGpp + H2O | - |
Homo sapiens | ppG + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview | Drosophila melanogaster |
More | MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HDDC3 | - |
Homo sapiens |
MESH1 | - |
Drosophila melanogaster |
MESH1 | - |
Homo sapiens |
metazoan SpoT homolog-1 | Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains | Drosophila melanogaster |
metazoan SpoT homolog-1 | Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains | Homo sapiens |
ppGpp hydrolase | - |
Drosophila melanogaster |
ppGpp hydrolase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | Mesh1 deletion impairs starvation resistance in Drosophila, overview | Drosophila melanogaster |
additional information | crucial residues for the ppGpp hydrolysis activity of Mesh1 are Arg24, Glu65, Asp66 and Asn126 | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.46 | - |
ppGpp | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
9.58 | - |
ppGpp | pH not specified in the publication, temperature not specified in the publication | Drosophila melanogaster |