Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sinorhizobium meliloti |
Crystallization (Comment) | Organism |
---|---|
structure to 2.8 A resolution, shows a buried, largely hydrophobic active site. Conserved residue Glu386 plays a role in recognition of the quaternary ammonium group of the choline substrate | Sinorhizobium meliloti |
Protein Variants | Comment | Organism |
---|---|---|
C54S | mutation in active site residue, significant decrease in activity | Sinorhizobium meliloti |
E386L | mutation in active site residue, significant decrease in activity | Sinorhizobium meliloti |
H104A | mutation in active site residue, significant decrease in activity | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
choline O-sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
2.1 | - |
phosphoryl choline | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
9.4 | - |
choline O-sulfate | mutant C54S, pH 7.6, 25°C | Sinorhizobium meliloti | |
13.3 | - |
choline O-sulfate | mutant H104A, pH 7.6, 25°C | Sinorhizobium meliloti | |
15 | - |
choline O-sulfate | mutant E386L, pH 7.6, 25°C | Sinorhizobium meliloti | |
19 | - |
4-nitrophenyl sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
50 | - |
4-nitrophenyl methylphosphate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti | |
67 | - |
4-nitrophenyl methylphosphonate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | O69787 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl methylphosphate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + methylphosphate | - |
? | |
4-nitrophenyl methylphosphonate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + phosphonate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + sulfate | - |
? | |
choline O-sulfate + H2O | - |
Sinorhizobium meliloti | choline + sulfate | - |
? | |
phosphoryl choline + H2O | - |
Sinorhizobium meliloti | choline + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BetC | - |
Sinorhizobium meliloti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
4-nitrophenyl methylphosphate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti | |
0.011 | - |
choline O-sulfate | mutant C54S, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.012 | - |
choline O-sulfate | mutant E386L, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.027 | - |
phosphoryl choline | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.035 | - |
choline O-sulfate | mutant H104A, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.1 | - |
4-nitrophenyl methylphosphonate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti | |
0.22 | - |
4-nitrophenyl sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
2.4 | - |
choline O-sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
4-nitrophenyl methylphosphate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti | |
0.0008 | - |
choline O-sulfate | mutant E386L, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.0012 | - |
choline O-sulfate | mutant C54S, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.0015 | - |
4-nitrophenyl methylphosphonate | wild-type, pH 6.0, 25°C | Sinorhizobium meliloti | |
0.0026 | - |
choline O-sulfate | mutant H104A, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.012 | - |
4-nitrophenyl sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
0.013 | - |
phosphoryl choline | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti | |
4.8 | - |
choline O-sulfate | wild-type, pH 7.6, 25°C | Sinorhizobium meliloti |