Literature summary for 3.1.6.4 extracted from

Rodriguez-Lopez, A.; Almeciga-Diaz, C.J.; Sanchez, J.; Moreno, J.; Beltran, L.; Diaz, D.; Pardo, A.; Ramirez, A.M.; Espejo-Mojica, A.J.; Pimentel, L.; Barrera, L.A.
Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris (2016), Sci. Rep., 6, 29329 .

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Application

Application	Comment	Organism
synthesis	removal of the native signal peptide and coexpression with human formylglycine-generating enzyme SUMF1 in Pichia pastoris allow an improvement of 4.5fold in the specific GALNS activity. Recombinant GALNS shows a high stability at 4°C, while the activity is markedly reduced at 37 and 45°C. Recombinant GALNS is taken-up by HEK-293 cells and human skin fibroblasts in a dose-dependent manner, without any additional protein or host modification	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P34059	-	-

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Release 2023.1 (January 2023)