Literature summary for 3.1.6.14 extracted from

Sikora, A.-S.; Hellec, C.; Carpentier, M.; Martinez, P.; Delos, M.; Denys, A.; Allain, F.
Tumour-necrosis factor-alpha induces heparan sulfate 6-O-endosulfatase1 (Sulf-1) expression in fibroblasts (2016), Int. J. Biochem. Cell Biol., 80, 57-65 .

Search for more literature for 3.1.6.14

Cloned(Commentary)

Cloned (Comment)	Organism
gene GNS, quantitative real-time PCR enzyme expression analysis	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	silencing the expression of Sulf-1 by RNA interference restores the responses induced by FGF-1, which indicates that TNF-alpha-mediated induction of the sulfatase results in alterations of HS biological properties	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell membrane	the heterodimeric enzyme is released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate	Homo sapiens	-	-
extracellular	the heterodimeric enzyme is released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P15586	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	Sulf-1 is synthesized as a about 125-kDa precursor that is cleaved by a furin-type proteinase. This process results in the formation of an active heterodimer, in which 75-kDa and 50-kDa subunits are linked by disulfide bonds	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast	-	Homo sapiens	-
MRC5-SV2 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl sulfate + H2O	fluorogenic substrate 4-MUS	Homo sapiens	4-methylumbelliferone + sulfate	-	?

Subunits

Subunits	Comment	Organism
More	Sulf-1 is synthesized as a about 125-kDa precursor that is cleaved by a furin-type proteinase. This process results in the formation of an active heterodimer, in which 75-kDa and 50-kDa subunits are linked by disulfide bonds. The enzyme is then released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate	Homo sapiens

Synonyms

Synonyms	Comment	Organism
heparan sulfate 6-O-endosulfatase	-	Homo sapiens
heparan sulfate 6-O-endosulfatase1	-	Homo sapiens
HS 6-O-endosulfatase	-	Homo sapiens
Sulf-1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	tumour-necrosis factor-alpha (TNF-alpha) induces heparan sulfate 6-O-endosulfatase1 (Sulf-1) expression in fibroblasts via a mechanism involving NF-kappaB, ERK1/2, and p38 MAPK. Cell stimulation with TNF-alpha is accompanied by the secretion of an active form of Sulf-1. Regulation of Sulf-1 expression by TNF-alpha, overview	up

General Information

General Information	Comment	Organism
additional information	TNF-alpha-treated cells show a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, suggesting a selective reaction of 6-O-desulfation. Cell stimulation with TNF-alpha is accompanied by the secretion of an active form of Sulf-1	Homo sapiens

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Release 2023.1 (January 2023)