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Literature summary for 3.1.6.13 extracted from

  • Bielicki, J.; Hopwood, J.J.; Wilson, P.J.; Anson, D.S.
    Recombinant human iduronate-2-sulphatase: correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme (1993), Biochem. J., 289, 241-246.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.13

Application

Application Comment Organism
medicine endocytosis of the recombinant enzyme by human fibroblasts Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Homo sapiens
NaCl
-
 Homo sapiens
phosphate
-
 Homo sapiens
SO42-
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate recombinant protein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 after endoglucosidase treatment, SDS-PAGE Homo sapiens
90000
-
 recombinant protein,SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dermatan sulfate + H2O Homo sapiens
-
 dermatan + sulfate
-
 ?
heparan sulfate + H2O Homo sapiens
-
 dermatan + sulfate
-
 ?
heparin + H2O Homo sapiens
-
 desulfated heparin + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein eight glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
-
 Homo sapiens
20.8
-
 recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate + H2O
-
 Homo sapiens dermatan + sulfate
-
 ?
heparan sulfate + H2O
-
 Homo sapiens dermatan + sulfate
-
 ?
heparin + H2O
-
 Homo sapiens desulfated heparin + sulfate
-
 ?
O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate + H2O
-
 Homo sapiens O-(alpha-L-idopyranosyluronic acid)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate + sulfate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.3 recombinant protein Homo sapiens