Literature summary for 3.1.4.52 extracted from

Hammer, B.K.; Bassler, B.L.
Distinct sensory pathways in Vibrio cholerae El Tor and classical biotypes modulate cyclic dimeric GMP levels to control biofilm formation (2009), J. Bacteriol., 191, 169-177.

Cloned(Commentary)

Cloned (Comment)	Organism
autoinducer-deficient Vibrio cholerae strain transformed with library of random Vibrio cholerae genomic fragments fused to a promoterless luciferase cassette. Verification of Quorum sensing (QS) regulation: wild-type Vibrio cholerae El and QS mutants are transformed with each candidate reporter plasmid, each candidate exhibits similar expression pattern in a luxO D47E strain (response regulator luxO) and a hapR strain (QS transcription factor HapR) that both simulate low cell density, indicating expression of each target gene is dependent on LuxO-P as well as HapR. VCA0681 is overexpressed in Vibrio cholerae luxO D47Eel: reduction of reporter expression. Deletion of either VCA0681 from the Vibrio cholerae El genome does not alter biofilm formation (transcriptional activator vps expression). Absolute level of vps expression in the (HapR minus) Vibrio cholerae El strain 100 times higher than that of wild-type (HapR minus) Vibrio cholerae C1 strain	Vibrio cholerae serotype O1
VCA0895 is overexpressed in Vibrio cholerae luxO D47Eel: growth inhibition, deletion of vca0895 from the Vibrio cholerae El genome does not alter biofilm formation (transcriptional activator vps expression)	Vibrio cholerae serotype O1

Cloned (Comment)

Organism

autoinducer-deficient Vibrio cholerae strain transformed with library of random Vibrio cholerae genomic fragments fused to a promoterless luciferase cassette. Verification of Quorum sensing (QS) regulation: wild-type Vibrio cholerae El and QS mutants are transformed with each candidate reporter plasmid, each candidate exhibits similar expression pattern in a luxO D47E strain (response regulator luxO) and a hapR strain (QS transcription factor HapR) that both simulate low cell density, indicating expression of each target gene is dependent on LuxO-P as well as HapR. VCA0681 is overexpressed in Vibrio cholerae luxO D47Eel: reduction of reporter expression. Deletion of either VCA0681 from the Vibrio cholerae El genome does not alter biofilm formation (transcriptional activator vps expression). Absolute level of vps expression in the (HapR minus) Vibrio cholerae El strain 100 times higher than that of wild-type (HapR minus) Vibrio cholerae C1 strain

Vibrio cholerae serotype O1

VCA0895 is overexpressed in Vibrio cholerae luxO D47Eel: growth inhibition, deletion of vca0895 from the Vibrio cholerae El genome does not alter biofilm formation (transcriptional activator vps expression)

Vibrio cholerae serotype O1

Protein Variants

Protein Variants	Comment	Organism
D289A	VCA0681, mutation in active site, eliminated VCA0681 repression of vpsL-lux, biofilm formation not repressed by mutant contrary to wild-type. Expression of the active-site mutant (D289A) in a luxO D47E strain has little effect on cyclic di-3',5'-guanylate levels	Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cyclic di-3',5'-guanylate + H2O	Vibrio cholerae serotype O1	-	5'phosphoguanylyl(3'-5')guanosine	-	?
cyclic di-3',5'-guanylate + H2O	Vibrio cholerae serotype O1 El Tor C6706	-	5'phosphoguanylyl(3'-5')guanosine	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-
Vibrio cholerae serotype O1 El Tor C6706	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclic di-3',5'-guanylate + H2O	-	Vibrio cholerae serotype O1	5'phosphoguanylyl(3'-5')guanosine	-	?
cyclic di-3',5'-guanylate + H2O	-	Vibrio cholerae serotype O1 El Tor C6706	5'phosphoguanylyl(3'-5')guanosine	-	?

Synonyms

Synonyms	Comment	Organism
HD-GYP	-	Vibrio cholerae serotype O1