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Literature summary for 3.1.4.39 extracted from

  • Nishimasu, H.; Okudaira, S.; Hama, K.; Mihara, E.; Dohmae, N.; Inoue, A.; Ishitani, R.; Takagi, J.; Aoki, J.; Nureki, O.
    Crystal structure of autotaxin and insight into GPCR activation by lipid mediators (2011), Nat. Struct. Mol. Biol., 18, 205-212.
    View publication on PubMed
Search for more literature for 3.1.4.39

Crystallization (Commentary)

Crystallization (Comment) Organism
ATX alone and in complex with LPAs with different acyl-chain lengths and saturations, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution Mus musculus

Protein Variants

Protein Variants Comment Organism
F249A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
F274A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
L213A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
L243A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
M512A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
N230A almost inactive mutant Mus musculus
W254A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus
Y306A the mutant shows decreased lysoPLD activity and differences in the fatty acid preferences compared to the wild-type enzyme Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Subunits

Subunits Comment Organism
More autotaxin domain arrangement and structure, overview Mus musculus

Synonyms

Synonyms Comment Organism
ATX
-
 Mus musculus
autotaxin
-
 Mus musculus
ENPP2
-
 Mus musculus
lysoPLD
-
 Mus musculus

General Information

General Information Comment Organism
additional information a loop region in the catalytic domain is a major determinant for the substrate specificity of the Enpp family enzymes, Asn230 is critical for the recognition of the common phosphate moieties, structure of the ATX active site in the free-form and with bound Zn2+, overview Mus musculus
physiological function autotaxin is a secreted lysophospholipase D that hydrolyzes lysophosphatidylcholine to generate lysophosphatidic acid, a lipid mediator that activates G protein-coupled receptors to evoke various cellular responses. The produced LPAs are delivered from the active site to cognate G protein-coupled receptors through a hydrophobic channel Mus musculus