Protein Variants | Comment | Organism |
---|---|---|
H226Q | the mutation impairs substrate hydrolysis, with hydrolysis of artificial substrates being disproportionately lower than that of lysophosphatidylcholine | Homo sapiens |
H420Q | the mutation does not inhibit the wild type migration response or hydrolytic activity | Homo sapiens |
H434Q | the mutation impairs substrate hydrolysis, with hydrolysis of artificial substrates being disproportionately lower than that of lysophosphatidylcholine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
FS-3 | wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.019 | - |
FS-3 | mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.066 | - |
lysophosphatidylcholine 16:0 | wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.069 | - |
lysophosphatidylcholine 18:1 | mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.073 | - |
lysophosphatidylcholine 18:1 | wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.089 | - |
lysophosphatidylcholine 18:0 | wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.094 | - |
lysophosphatidylcholine 16:0 | mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.105 | - |
lysophosphatidylcholine 18:1 | mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.115 | - |
lysophosphatidylcholine 18:0 | mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.12 | - |
lysophosphatidylcholine 16:0 | mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.126 | - |
lysophosphatidylcholine 18:1 | mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.126 | - |
lysophosphatidylcholine 16:0 | mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.27 | - |
lysophosphatidylcholine 18:0 | mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.7 | - |
4-nitrophenyl-TMP | wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
4.8 | - |
4-nitrophenyl-TMP | mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-TMP + H2O | - |
Homo sapiens | ? | - |
? | |
FS-3 + H2O | fluorogenic lysoPLD substrate | Homo sapiens | 2-(6-hydroxy-3-oxo-9a,10a-dihydro-3H-xanthen-9-yl)-5-[(18-hydroxy-15-oxo-3,6,9,12-tetraoxa-16-azaoctadec-1-yl)carbamoyl]benzoic acid + (2R)-2-hydroxy-3-(phosphonooxy)propyl 6-[(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]benzoyl)amino]hexanoate | - |
? | |
lysophosphatidylcholine 16:0 + H2O | - |
Homo sapiens | ? | - |
? | |
lysophosphatidylcholine 18:0 + H2O | - |
Homo sapiens | ? | - |
? | |
lysophosphatidylcholine 18:1 + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATX | - |
Homo sapiens |
autotaxin | - |
Homo sapiens |
NPP2 | - |
Homo sapiens |