Application | Comment | Organism |
---|---|---|
analysis | analysis of heteroduplex and superhelical DNA, quantitation of nucleic acid hybridization | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-dAMP | 50% inhibition by 0.085 mM dAMP | Aspergillus oryzae | |
5'-dATP | 50% inhibition by 0.001 mM dATP | Aspergillus oryzae | |
phosphate | 50% inhibition by 2 mM sodium phosphate | Aspergillus oryzae | |
sodium bisphosphate | 50% inhibition by 0.02 mM sodium bisphosphate | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Aspergillus oryzae | |
Hg2+ | maximal activity at 0.01-1 mM at pH 3.5-5.0 | Aspergillus oryzae | |
Zn2+ | - |
Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Aspergillus oryzae | 75000 times more active on single-stranded than on native DNA | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.7 | - |
purified enzyme, heat-denaturated DNA as substrate | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | 75000 times more active on single-stranded than on native DNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
dsDNA + H2O | activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S1 nuclease | - |
Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | - |
Aspergillus oryzae |
4.5 | - |
in 10 mM NaCl | Aspergillus oryzae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
5'-dATP | - |
Aspergillus oryzae | |
0.085 | - |
5'-dAMP | - |
Aspergillus oryzae |